Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.12.08 Master Mix
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8.12.08 Master Mix
| Reagent | Volume/Amount |
| Colony Suspension | 9.5 μl (not added to master, though) |
| Taq | 0.95 μl |
| Primer BT2736 | 7.6 μl 1 μM Stock |
| Primer BT2737 | 7.6 μl 1 μM Stock |
| dNTPs | 1.9 μl 10 mM Stock |
| DMSO | 4.75 μl |
| Buffer | 9.5 μl |
| Mg2+ | 4.75 μl |
| dH20 | 48.45 μl |
| Total | 95 μl |
Aliquotted 9 μl of to each of 9 tubes, then added 1 μl appropriate colony suspension.
