Matt Gethers/CRI, Thailand/Labwork/HmgR Isolation Protocol/7.31.08 Gel Results
7.31.08 SDS-PAGE Induction Assay
|Lane 1||Lane 2||Lane 3||Lane 4||Lane 5||Lane 6||Lane 7||Lane 8||Lane 9|
|5 μl Unstained protein marker||5 μl P5/Pellet/-IPTG||5 μl P5/Pellet/+IPTG||5 μl P5/Supernatant/-IPTG||5 μl P5/Supernatant/+IPTG||5 μl P6/Supernatant/-IPTG||5 μl P6/Supernatant/+IPTG||5 μl P1/Supernatant/-IPTG||5 μl P1/Supernatant/+IPTG|
Ran for 1 hour at 200 V. Stained for 40 minutes in 0.3% Coomassie, destained for ~40 minutes in destaining solution. Lanes 2 and 3 were faint, but 4-7 were essentially identical. It looks like I failed to get induction again. Lanes 8 and 9 clearly show induction with a pronounced band at -28 kDa.