Matt Gethers/CRI, Thailand/Labwork/HmgR Isolation Protocol/7.31.08 Gel Results

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7.31.08 SDS-PAGE Induction Assay

Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9
5 μl Unstained protein marker5 μl P5/Pellet/-IPTG5 μl P5/Pellet/+IPTG5 μl P5/Supernatant/-IPTG5 μl P5/Supernatant/+IPTG5 μl P6/Supernatant/-IPTG5 μl P6/Supernatant/+IPTG5 μl P1/Supernatant/-IPTG5 μl P1/Supernatant/+IPTG

Ran for 1 hour at 200 V. Stained for 40 minutes in 0.3% Coomassie, destained for ~40 minutes in destaining solution. Lanes 2 and 3 were faint, but 4-7 were essentially identical. It looks like I failed to get induction again. Lanes 8 and 9 clearly show induction with a pronounced band at -28 kDa.

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