Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.11.08 Gel Results

From OpenWetWare

Jump to: navigation, search

8.11.08 PCR Gel Results

Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8
5 μl λ ladder5 μl PCR product pIs001.P7 5 μl PCR product pIs001.P8 5 μl PCR product pIs001.L8 '4'5 μl PCR product pIs001.L8 '5'5 μl PCR product pIs001.L8 '9'5 μl PCR product pIs001.L8 '10'genomic DNA positive control

Lanes 2 and 3 (corresponding to P7 and P8 respectively) yielded products at ~4200 bp - Way Off! None of the colony PCRs yielded any product and the positive control worked properly with a band at ~800. Something's gone terribly wrong with the preps.

Personal tools