Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.7.08 Master Mix

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8.7.08 Master Mix

Reagent Volume/Amount
Colony Suspension9.5 μl (not added to master, though)
Taq0.95 μl
Primer BT27367.6 μl 1 μM Stock
Primer BT27377.6 μl 1 μM Stock
dNTPs1.9 μl 10 mM Stock
DMSO4.75 μl
Buffer 9.5 μl
Mg2+ 4.75 μl
dH2048.45 μl
Total95 μl

Aliquotted 9 μl of to each of 9 tubes, then added 1 μl appropriate colony suspension.

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