Matt Gethers/CRI, Thailand/Labwork/PCRs/PCR Pfu Template

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Contents

PCR PFU Template

Rxn Mix

Reagent Volume/Amount
Paeru Genomic DNA Template0.5 μl from So Pa Pan stock
Pfu0.5 μl
4 μl 1 μM Stock
4 μl 1 μM Stock
dNTPs1 μl 10 mM Stock
DMSO 2.5 μl
Buffer S5 μl
dH2032.5 μl
Total50 μl


Rxn Conditions

Annealing Temperature: oC

Extension Time:

Cycle

Step Temperature Duration Notes
Initial Denaturation95oC3 minutes
Repeat Cyclic Steps 35x
Cyclic Denaturation94oC40 Seconds
Cyclic AnnealingoC40 Seconds
Cyclic Extension72oC minutes
Repeat Cyclic Steps 35x
Final Extension72oC10 minutes

Run Notes

References

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