Matt Gethers/CRI, Thailand/Labwork/PCRs/PCR Supermix Template

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Contents

PCR Supermix Template

Rxn Mix

Reagent Volume/Amount
DNA Template1.0 μl from Colony Suspension
1.2 μl 1 μM Stock
1.2 μl 1 μM Stock
2x PCR Master7.5 μl
H204.1 μl
Total15 μl

Rxn Conditions

Annealing Temperature: oC

Extension Time:

Cycle (Taq)

Step Temperature Duration Notes
Initial Denaturation95oC2 minutes
Repeat Cyclic Steps 35x
Cyclic Denaturation94oC30 Seconds
Cyclic AnnealingoC30 Seconds
Cyclic Extension72oC minute
Repeat Cyclic Steps 35x
Final Extension72oC10 minutes


Run Notes

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