Matt Gethers/CRI, Thailand/Labwork/PCRs/PCR Taq Template

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Contents

PCR Taq Template

Rxn Mix

Reagent Volume/Amount
Paeru Genomic DNA Template0.5 μl
Taq0.5 μl
4 μl 1 μM Stock
4 μl 1 μM Stock
dNTPs1 μl 10 mM Stock
DMSO 2.5 μl
Buffer 5 μl
Mg2+ 2.5 μl
dH2030 μl
Total50 μl

Rxn Conditions

Annealing Temperature:

Extension Time:

Cycle (Taq)

Step Temperature Duration Notes
Initial Denaturation95oC2 minutes
Repeat Cyclic Steps 35x
Cyclic Denaturation94oC30 Seconds
Cyclic AnnealingoC30 SecondsUnsure of best temp
Cyclic Extension72oC minute
Repeat Cyclic Steps 35x
Final Extension72oC10 minutes

Run Notes

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