Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence and Directionality of HmgR Downstream Fragment in pUC18/7.24.08 Master Mix

From OpenWetWare

Jump to: navigation, search

7.24.08 PCR Master Mix

Reagent Volume/Amount
Colony Suspension0 μl
Taq1.63 μl
M13 for13.04 μl 1 μM Stock
BT118813.04 μl 1 μM Stock
dNTPs3.26 μl 10 mM Stock
DMSO 8.15 μl
Buffer 16.3 μl
Mg2+ 8.15 μl
dH2083.13 μl
Total146.7 μl

Aliquot 9 μl to each of 16 tubes, then add 1 μl colony suspension to each.

Personal tools