Matt Gethers/CRI, Thailand/Labwork/Phosphatase Treatment

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Alkaline Phosphatase Treatment (SAP Enzyme)

Reagent Volume Notes
Digested Vector8.5 μl
SAP Buffer1 μl
SAP Enzyme0.5 μl
Total10 μl

Incubate mix at 37oC for 30 minutes, then inactivate at 65oC for 15 minutes.

Run Notes


Ran protocol as written to dephosphorylate pKn002.P1 cut with SphI and HindIII.

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