Matt Gethers/CRI, Thailand/Labwork/Polyacrylamide Gels/Mayuree's Mix

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Reagent Volume (Separation Layer) Volume (Stacking Layer) Notes
MilliQ3.48 ml3.09 ml
1.5 M Tris-Cl, pH 8.82.5 mln/aDon't use this in the stacking layer; kept in cabinets above gel boxes
0.5 M Tris-Cl, pH 6.8n/a1.25 mlDon't use this in the separation layer;kept in cabinets above gel boxes
10% SDS100 μl50 μlkept in cabinets above gel boxes
40% Bis:Acrylamide3.75 ml0.5 mlkept in fridge in adjacent bay
TEMED15 μl10 μlAdd this reagent last, then pour gel immediately after;kept in cabinets above gel boxes
10% APS150 μl100 μlAdd this reagent last, then pour gel immediately after; Keep an aliquot in the -20, 0.1 g in 1 ml H20
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