Matt Gethers/CRI, Thailand/Labwork/Polyacrylamide Gels/Week of 6.30.08

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Week of 6.30.08

7.3.08

First gel - ran with Mayuree. Made up mix of regents according to her protocol (about 2x). Set the gel 2 days before running it - kept in -4 until used on Thursday. Used Mini Isolation protocol to produce lysate and ran 5 μl in each lane.

Induction looks good. Our band (~28 kDa) is where it should be and only appears significantly in the induced lanes. Presence seems to be balanced between pellet and cytosolic fraction.

Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9
5 μl Unstained protein marker5 μl uninduced cell sample 1, pellet fraction5 μl induced cell sample 1, pellet fraction5 μl uninduced cell sample 2, pellet fraction5 μl induced cell sample 2, pellet fraction5 μl uninduced cell sample 1, supernatant5 μl induced cell sample 1, supernatant5 μl uninduced cell sample 2, supernatant5 μl induced cell sample 2, supernatant
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