Matthew Loper: M13 Renovation
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Gene | Current Function | Potential Re-Engineering Ideas |
---|---|---|
1 | Assembly | It would be interesting to test how much p1, p4, and p11 depend on each other. Is it possible to modify one gene without disrupting the function of the other two? I would also like to vary the number of secretion channels to test whether increasing the number of channels decreases the length of the phage's life cycle. |
2 | + strand DNA replication | Again, this gene overlaps with another gene with a different function (gene 10). It may be useful to seperate p2 and p10 so that they can be changed independently. Perhaps we could make + strand replication faster |
3 | phage tail protein which is initial point of infection | Increase binding affinity so that in a population of bacteria there is a higher rate of M13 infection |
4 | Assembly | Similar function to p1 |
5 | Binds ssDNA/make protein DNA complexes | Again, I see potential to decrease the phage life cycle/create greater efficiency. Altering p5 could increase the rate of replication |
6 | phage tail protein | This tail protein does not seem to be as necessary to the infection process as p3 so it may be a convenient location to add a GFP fusion for labelling purposes. |
7 | phage head protein | If GFP fusion on p6 was not successful this is another good candidate for labeling purposes. |
8 | Phage coat protein (2700 copies) | Currently flexible in order to adjust size to genome size. Modify rigidity in order to essentially cap genome size at desired modified size as a check on inadvertent modifications and mutations. |
9 | Blunt end of phage | Make this gene like p3...can we change side selectivity of M13? Would M13 be able to infect using both ends? How would this affect the virus? |
10 | + strand accumulation | Modulate from p2. Can we increase strand accumulation in host? How does this affect replication rate? |
11 | Assembly | Same as p1 |