McClean:LB2

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Contents

Overview

Liquid LB media

Reagents

  • Bacto tryptone
  • Bacto yeast extract
  • NaCl
  • MiliQ water

Protocol

  • Begin with approximately 800mls of MiliQ water
  • Weigh out and add ingredients with constant stirring:
    • Bacto tryptone 10g
    • Bacto yeast extract 5g
    • NaCl 5g
  • Bring up to 1L with MiliQ water
  • Autoclave


Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

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References

Contact

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