McKinney:SodiumOleate

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Sodium oleate solution

This is an additive to media used to culture M. tuberculosis.

Ingredients

(make 500mL)

  • 12.5mL 2M NaOH
  • 6.0mL oleic acid (=5g, since oleic acid specific gravity is 1g/1.2mL)
  • 481.5mL water

Protocol

Combine the above ingredients in a 1L flask. Stir with a magnetic stirbar until completely dissolved. Filter sterilize through a 0.22μm pore filter. Store at room temperature wrapped in aluminum foil (oleic acid is light sensitive).

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