Sodium oleate solution
This is an additive to media used to culture M. tuberculosis.
- 12.5mL 2M NaOH
- 6.0mL oleic acid (=5g, since oleic acid specific gravity is 1g/1.2mL)
- 481.5mL water
Combine the above ingredients in a 1L flask. Stir with a magnetic stirbar until completely dissolved. Filter sterilize through a 0.22μm pore filter. Store at room temperature wrapped in aluminum foil (oleic acid is light sensitive).