Mike Barnkob:Protocols/Bacterial/Streaking

From OpenWetWare

Jump to: navigation, search

Front page

Contents

Streaking plates

How to streak plates with stab or bacterial cultures.

Reagents

Protocol

  1. Use sterile loop and dip into stab culture, bacterial plate or glycerol stock
    Note: If using re-usable loop, vigorously sterilize loop with Bunsen burner every time you use it. Likewise, if using plastic loops, use a new one for each action.
  2. Gently streak loop over LB plate following the pattern shown in 1. Using a new loop, drag through the first streak-pattern, as shown in 2. Do the same for the third pattern.
    Note: Using left hand keep lid raised above plate – this will reduce contamination getting onto the plate.
    Note: Use a new loop for each streak.
    Note: Only touch the surface of the plate, do not dig into the agar.
  3. Label plates with name, date, type of plate, bacteria / plasmid.
  4. Incubate plate overnight (12-18 hours) at 37°C and then proceed to create a liquid culture.
    Note: Wrap plates in parafilm to avoid drying of plate.

References

Personal tools