Mimulus:Seed Sterilization
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Seed Sterilization
Supplies
- 70% Ethanol
- 20% Bleach
- Sterile Water
- Seeds
Methods
- Put seeds in an Eppendorf tube
- Add 200uL of 70% EtOH to tube
- Immediately add 600 uL of Water. Do only 2 tubes at a time, because the alcohol treatment is harsh and might affect germination if is left on seeds too long
- Immediately suck off 600 uL of supernatant
- Repeat steps 3 and 4 four times with water, not ethanol, to wash
- Set aside tubes and repeat steps 1 to 5 for all the remaining tubes
- Add 200 uL of 20% bleach to each tube
- Immediatly remove bleach solution
- Add 600 uL of water
- Suck off 600 uL of the supernatant
- Repeat steps 9 and 10 four or five times to wash