Muratore:Materials/Plasmids/pTXB1+BSA

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Information

BSA cloned into the NheI and SapI sites of pTXB1

Map

Image:pTXB1-BSA.bmp

Sequence

    1 TCACTATAGG GGAATTGTGA GCGGATAACA ATTCCCCTCT AGAAATAATT
   51 TTGTTTAACT TTAAGAAGGA GATATACATA TGGCTAGCAA GTGGGTGACT
  101 TTTATTTCTC TTCTCCTTCT CTTCAGCTCT GCTTATTCCA GGGGTGTGTT
  151 TCGTCGAGAT AAACACAAGA GTGAGATTGC TCATCGGTTT AAAGATTTGG
  201 GAGAAGAACA TTTTAAAGGC CTGGTACTGA TTGCCTTTTC TCAGTATCTC
  251 CAGCAGTGTC CATTTGATGA GCATGTAAAA TTAGTGAACG AACTAACTGA
  301 GTTTGCAAAA ACATGTGTTG CTGATGAGTC CCATGCCGGC TGTGAAAAGT
  351 CACTTCACAC TCTCTTTGGA GATGAATTGT GTAAAGTTGC ATCCCTTCGT
  401 GAAACCTATG GTGACATGGC TGACTGCTGT GCGAAACAAG AGCCTGAAAG
  451 AAATGAATGC TTCCTGAGCC ACAAAGATGA TAGCCCAGAC CTCCCTAAAT
  501 TGAAACCAGA CCCCAATACT TTGTGTGATG AGTTTAAGGC AGATGAAAAG
  551 AAGTTTTGGG GAAAATACCT ATACGAAATT GCTAGAAGAC ATCCCTACTT
  601 TTATGCACCA GAACTCCTTT ACTATGCTAA TAAATATAAT GGAGTTTTTC
  651 AAGAATGCTG CCAAGCTGAA GATAAAGGTG CCTGCCTGCT ACCAAAGATT
  701 GAAACTATGA GAGAAAAAGT ACTGACTTCA TCTGCCAGAC AGAGACTCAG
  751 GTGTGCCAGT ATTCAAAAAT TTGGAGAAAG AGCTTTAAAA GCATGGTCAG
  801 TAGCTCGCCT GAGCCAGAAA TTTCCCAAGG CTGAGTTTGT AGAAGTTACC
  851 AAGCTAGTGA CAGATCTCAC AAAAGTCCAC AAGGAATGCT GCCATGGTGA
  901 CCTACTTGAA TGCGCAGATG ACAGGGCAGA TCTTGCCAAG TACATATGTG
  951 ATAATCAAGA TACAATCTCC AGTAAACTGA AGGAATGCTG TGATAAGCCT
 1001 TTGTTGGAAA AATCCCACTG CATTGCTGAG GTGGAAAAAG ATGCCATACC
 1051 TGAAAACCTG CCCCCATTAA CTGCTGACTT TGCTGAAGAT AAGGATGTTT
 1101 GCAAAAACTA TCAGGAAGCA AAAGATGCCT TCCTGGGCTC GTTTTTGTAT
 1151 GAATATTCAA GAAGGCATCC TGAATATGCT GTCTCAGTGC TATTGAGACT
 1201 TGCCAAGGAA TATGAAGCCA CACTGGAGGA ATGCTGTGCC AAAGATGATC
 1251 AACATGCATG CTATTCCACA GTGTTTGACA AACTTAAGCA TCTTGTGGAT
 1301 GAGCCACAGA ATTTAATCAA ACAAAACTGT GACCAATTCG AAAAACTTGG
 1351 AGAGTATGGA TTCCAAAATG AGCTCATAGT TCGTTACACC AGGAAAGTAC
 1401 CCCAAGTGTC AACTCCAACT CTCGTGGAGG TTTCAAGAAG CCTAGGAAAA
 1451 GTGGGTACTA GGTGTTGTAC AAAGCCGGAA TCAGAAAGAA TGCCCTGTGC
 1501 TGAAGACTAT CTGAGCTTGA TCCTGAACCG GTTGTGCGTG CTGCATGAGA
 1551 AGACACCAGT GAGTGAAAAA GTCACCAAGT GCTGCACAGA GTCATTGGTG
 1601 AACAGACGGC CATGTTTCTC TGCTCTGACA CCTGATGAAA CATATGTACC
 1651 CAAAGCCTTT GATGAGAAAT TGTTCACCTT CCATGCAGAT ATATGCACAC
 1701 TTCCCGATAC TGAGAAACAA ATCAAGAAAC AAACTGCACT TGTTGAGCTG
 1751 TTGAAACACA AGCCCAAGGC AACAGAGGAA CAACTGAAAA CCGTCATGGA
 1801 GAATTTTGTG GCTTTTGTAG GCAAGTGCTG TGCAGCTGAT GACAAAGAGG
 1851 CCTGCTTTGC TGTGGAGGGT CCAAAACTTG TTGTTTCAAC TCAAACAGCC
 1901 TTAGCCTGCA TCACGGGAGA TGCACTAGTT GCCCTACCCG AGGGCGAGTC
 1951 GGTACGCATC GCCGACATCG TGCCGGGTGC GCGGCCCAAC AGTGACAACG
 2001 CCATCGACCT GAAAGTCCTT GACCGGCATG GCAATCCCGT GCTCGCCGAC
 2051 CGGCTGTTCC ACTCCGGCGA GCATCCGGTG TACACGGTGC GTACGGTCGA
 2101 AGGTCTGCGT GTGACGGGCA CCGCGAACCA CCCGTTGTTG TGTTTGGTCG
 2151 ACGTCGCCGG GGTGCCGACC CTGCTGTGGA AGCTGATCGA CGAAATCAAG
 2201 CCGGGCGATT ACGCGGTGAT TCAACGCAGC GCATTCAGCG TCGACTGTGC
 2251 AGGTTTTGCC CGCGGAAAAC CCGAATTTGC GCCCACAACC TACACAGTCG
 2301 GCGTCCCTGG ACTGGTGCGT TTCTTGGAAG CACACCACCG AGACCCGGAC
 2351 GCCCAAGCTA TCGCCGACGA GCTGACCGAC GGGCGGTTCT ACTACGCGAA
 2401 AGTCGCCAGT GTCACCGACG CCGGCGTGCA GCCGGTGTAT AGCCTTCGTG
 2451 TCGACACGGC AGACCACGCG TTTATCACGA ACGGGTTCGT CAGCCACGCT
 2501 ACTGGCCTCA CCGGTCTGAA CTCAGGCCTC ACGACAAATC CTGGTGTATC
 2551 CGCTTGGCAG GTCAACACAG CTTATACTGC GGGACAATTG GTCACATATA
 2601 ACGGCAAGAC GTATAAATGT TTGCAGCCCC ACACCTCCTT GGCAGGATGG
 2651 GAACCATCCA ACGTTCCTGC CTTGTGGCAG CTTCAATGAC TGCAGGAAGG
 2701 GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA
 2751 CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
 2801 AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATAAC TACGTCAGGT
 2851 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA
 2901 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC
 2951 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC
 3001 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC
 3051 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG
 3101 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT
 3151 CGCCCCGAAG AACGTTCTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG
 3201 TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC
 3251 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA
 3301 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT
 3351 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG
 3401 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT
 3451 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA
 3501 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT
 3551 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG
 3601 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC
 3651 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG
 3701 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT
 3751 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT
 3801 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG
 3851 TTTACTCATA TATACTTTAG ATTGATTTAC CCCGGTTGAT AATCAGAAAA
 3901 GCCCCAAAAA CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT
 3951 AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT
 4001 TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC
 4051 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA
 4101 AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA
 4151 TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT
 4201 GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT
 4251 TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA
 4301 AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA
 4351 CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTAAAAGGAT
 4401 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG
 4451 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT
 4501 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA
 4551 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC
 4601 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC
 4651 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC
 4701 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
 4751 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT
 4801 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT
 4851 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG
 4901 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC
 4951 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC
 5001 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
 5051 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC
 5101 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT
 5151 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT
 5201 TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
 5251 TCAGTGAGCG AGGAAGCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG
 5301 CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA
 5351 TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT
 5401 TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG
 5451 CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC
 5501 TGCGGTAAAG CTCATCAGCG TGGTCGTGCA GCGATTCACA GATGTCTGCC
 5551 TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG
 5601 GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA
 5651 CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG
 5701 ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC
 5751 CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC
 5801 GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCCGAA CGCCAGCAAG
 5851 ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC
 5901 GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT
 5951 GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG
 6001 CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC
 6051 TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA
 6101 TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC
 6151 ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC
 6201 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
 6251 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
 6301 CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT
 6351 TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC
 6401 CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA
 6451 TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA
 6501 CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA
 6551 TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG
 6601 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG
 6651 CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA
 6701 CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG
 6751 CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT
 6801 CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA
 6851 AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG
 6901 GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA
 6951 GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC
 7001 GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC
 7051 GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA
 7101 TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG
 7151 TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA
 7201 AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC
 7251 CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC
 7301 CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT
 7351 GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC
 7401 TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC
 7451 CGCAAGGAAT GGTGCATGCC GGCATGCCGC CCTTTCGTCT TCAAGAATTA
 7501 ATTCCCAATT CCCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC
 7551 TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG
 7601 GACAAATCCG CCGGGAGCGG ATTTGAACGT TGCGAAGCAA CGGCCCGGAG
 7651 GGTGGCGGGC AGGACGCCCG CCATAAACTG CCAGGAATTA ATTCCCCAGG
 7701 CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT
 7751 CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG
 7801 CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC
 7851 CCGCCATAAA CTGCCAGGAA TTAATTCCCC AGGCATCAAA TAAAACGAAA
 7901 GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA
 7951 ACGCTCTCCT GAGTAGGACA AATCCGCCGG GAGCGGATTT GAACGTTGCG
 8001 AAGCAACGGC CCGGAGGGTG GCGGGCAGGA CGCCCGCCAT AAACTGCCAG
 8051 GAATTAATTC CCCAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT
 8101 GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT CCTGAGTAGG
 8151 ACAAATCCGC CGGGAGCGGA TTTGAACGTT GCGAAGCAAC GGCCCGGAGG
 8201 GTGGCGGGCA GGACGCCCGC CATAAACTGC CAGGAATTAA TTCCCCAGGC
 8251 ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC
 8301 TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGGGAGC
 8351 GGATTTGAAC GTTGCGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC
 8401 CGCCATAAAC TGCCAGGAAT TGGGGATCGG AATTAATTCC CGGTTTAAAC
 8451 CGGGGATCTC GATCCCGCGA AATTAATACG AC
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