Muratore:Materials/Plasmids/pTXB1His

From OpenWetWare

Jump to: navigation, search

Information

pTXB1 with a His8 tag after the chitin binding domain.

See User:Kathryn_Muratore/Notebook/AU_CHEM-570_lab_prep/2011/06/13 to User:Kathryn_Muratore/Notebook/AU_CHEM-570_lab_prep/2011/06/15 for cloning details.

Map

Image:pTXB1-His.bmp

Sequence

    1 AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATAAC TACGTCAGGT
   51 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA
  101 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC
  151 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC
  201 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC
  251 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG
  301 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT
  351 CGCCCCGAAG AACGTTCTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG
  401 TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC
  451 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA
  501 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT
  551 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG
  601 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT
  651 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA
  701 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT
  751 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG
  801 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC
  851 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG
  901 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT
  951 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT
 1001 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG
 1051 TTTACTCATA TATACTTTAG ATTGATTTAC CCCGGTTGAT AATCAGAAAA
 1101 GCCCCAAAAA CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT
 1151 AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT
 1201 TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC
 1251 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA
 1301 AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA
 1351 TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT
 1401 GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT
 1451 TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA
 1501 AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA
 1551 CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTAAAAGGAT
 1601 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG
 1651 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT
 1701 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA
 1751 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC
 1801 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC
 1851 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC
 1901 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
 1951 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT
 2001 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT
 2051 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG
 2101 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC
 2151 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC
 2201 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
 2251 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC
 2301 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT
 2351 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT
 2401 TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
 2451 TCAGTGAGCG AGGAAGCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG
 2501 CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA
 2551 TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT
 2601 TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG
 2651 CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC
 2701 TGCGGTAAAG CTCATCAGCG TGGTCGTGCA GCGATTCACA GATGTCTGCC
 2751 TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG
 2801 GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA
 2851 CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG
 2901 ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC
 2951 CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC
 3001 GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCCGAA CGCCAGCAAG
 3051 ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC
 3101 GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT
 3151 GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG
 3201 CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC
 3251 TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA
 3301 TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC
 3351 ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC
 3401 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
 3451 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
 3501 CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT
 3551 TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC
 3601 CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA
 3651 TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA
 3701 CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA
 3751 TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG
 3801 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG
 3851 CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA
 3901 CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG
 3951 CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT
 4001 CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA
 4051 AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG
 4101 GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA
 4151 GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC
 4201 GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC
 4251 GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA
 4301 TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG
 4351 TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA
 4401 AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC
 4451 CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC
 4501 CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT
 4551 GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC
 4601 TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC
 4651 CGCAAGGAAT GGTGCATGCC GGCATGCCGC CCTTTCGTCT TCAAGAATTA
 4701 ATTCCCAATT CCCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC
 4751 TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG
 4801 GACAAATCCG CCGGGAGCGG ATTTGAACGT TGCGAAGCAA CGGCCCGGAG
 4851 GGTGGCGGGC AGGACGCCCG CCATAAACTG CCAGGAATTA ATTCCCCAGG
 4901 CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT
 4951 CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG
 5001 CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC
 5051 CCGCCATAAA CTGCCAGGAA TTAATTCCCC AGGCATCAAA TAAAACGAAA
 5101 GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA
 5151 ACGCTCTCCT GAGTAGGACA AATCCGCCGG GAGCGGATTT GAACGTTGCG
 5201 AAGCAACGGC CCGGAGGGTG GCGGGCAGGA CGCCCGCCAT AAACTGCCAG
 5251 GAATTAATTC CCCAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT
 5301 GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT CCTGAGTAGG
 5351 ACAAATCCGC CGGGAGCGGA TTTGAACGTT GCGAAGCAAC GGCCCGGAGG
 5401 GTGGCGGGCA GGACGCCCGC CATAAACTGC CAGGAATTAA TTCCCCAGGC
 5451 ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC
 5501 TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGGGAGC
 5551 GGATTTGAAC GTTGCGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC
 5601 CGCCATAAAC TGCCAGGAAT TGGGGATCGG AATTAATTCC CGGTTTAAAC
 5651 CGGGGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT
 5701 GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG
 5751 GAGATATACA TATGGCTAGC TCGCGAGTCG ACGGCGGCCG CGAATTCCTC
 5801 GAGGGCTCTT CCTGCATCAC GGGAGATGCA CTAGTTGCCC TACCCGAGGG
 5851 CGAGTCGGTA CGCATCGCCG ACATCGTGCC GGGTGCGCGG CCCAACAGTG
 5901 ACAACGCCAT CGACCTGAAA GTCCTTGACC GGCATGGCAA TCCCGTGCTC
 5951 GCCGACCGGC TGTTCCACTC CGGCGAGCAT CCGGTGTACA CGGTGCGTAC
 6001 GGTCGAAGGT CTGCGTGTGA CGGGCACCGC GAACCACCCG TTGTTGTGTT
 6051 TGGTCGACGT CGCCGGGGTG CCGACCCTGC TGTGGAAGCT GATCGACGAA
 6101 ATCAAGCCGG GCGATTACGC GGTGATTCAA CGCAGCGCAT TCAGCGTCGA
 6151 CTGTGCAGGT TTTGCCCGCG GAAAACCCGA ATTTGCGCCC ACAACCTACA
 6201 CAGTCGGCGT CCCTGGACTG GTGCGTTTCT TGGAAGCACA CCACCGAGAC
 6251 CCGGACGCCC AAGCTATCGC CGACGAGCTG ACCGACGGGC GGTTCTACTA
 6301 CGCGAAAGTC GCCAGTGTCA CCGACGCCGG CGTGCAGCCG GTGTATAGCC
 6351 TTCGTGTCGA CACGGCAGAC CACGCGTTTA TCACGAACGG GTTCGTCAGC
 6401 CACGCTACTG GCCTCACCGG TCTGAACTCA GGCCTCACGA CAAATCCTGG
 6451 TGTATCCGCT TGGCAGGTCA ACACAGCTTA TACTGCGGGA CAATTGGTCA
 6501 CATATAACGG CAAGACGTAT AAATGTTTGC AGCCCCACAC CTCCTTGGCA
 6551 GGATGGGAAC CATCCAACGT TCCTGCCTTG TGGCAGCTTC AACACCACCA
 6601 TCATCATCAC CACCACTGAC TGCAGGAAGG GGATCCGGCT GCTAACAAAG
 6651 CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA
 6701 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
Personal tools