Muratore:Materials/Plasmids/pTXB1His+BSA

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Information

BSA cloned into the NheI and SapI sites of pTXB1His

Map

Image:pTXB1-His-BSA.bmp

Sequence

    1 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA
   51 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG
  101 GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA
  151 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC
  201 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTCTCCA
  251 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT
  301 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG
  351 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG
  401 ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC
  451 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT
  501 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG
  551 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT
  601 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC
  651 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA
  701 GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA
  751 ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC
  801 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG
  851 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT
  901 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA
  951 TTGATTTACC CCGGTTGATA ATCAGAAAAG CCCCAAAAAC AGGAAGATTG
 1001 TATAAGCAAA TATTTAAATT GTAAACGTTA ATATTTTGTT AAAATTCGCG
 1051 TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG
 1101 CAAAATCCCT TATAAATCAA AAGAATAGCC CGAGATAGGG TTGAGTGTTG
 1151 TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC
 1201 AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC
 1251 ACCCAAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA
 1301 ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC
 1351 GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT
 1401 GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA
 1451 ATGCGCCGCT ACAGGGCGCG TAAAAGGATC TAGGTGAAGA TCCTTTTTGA
 1501 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
 1551 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG
 1601 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
 1651 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT
 1701 TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA
 1751 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
 1801 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
 1851 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA
 1901 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
 1951 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG
 2001 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
 2051 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
 2101 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
 2151 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
 2201 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC
 2251 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC
 2301 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCTATG
 2351 GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA
 2401 CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC
 2451 CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT
 2501 TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC
 2551 ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT
 2601 GGTCGTGCAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG
 2651 TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT
 2701 GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG
 2751 GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC
 2801 ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA
 2851 GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC
 2901 AGGGTCAATG CCAGCCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC
 2951 GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG
 3001 ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA
 3051 GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA
 3101 ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA
 3151 GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG
 3201 AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGAGA TCCCGGTGCC
 3251 TAATGAGTGA GCTAACTTAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
 3301 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG
 3351 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC AGGGTGGTTT TTCTTTTCAC
 3401 CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG CCCTGAGAGA
 3451 GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT
 3501 TTGATGGTGG TTAACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC
 3551 GTATCCCACT ACCGAGATAT CCGCACCAAC GCGCAGCCCG GACTCGGTAA
 3601 TGGCGCGCAT TGCGCCCAGC GCCATCTGAT CGTTGGCAAC CAGCATCGCA
 3651 GTGGGAACGA TGCCCTCATT CAGCATTTGC ATGGTTTGTT GAAAACCGGA
 3701 CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA ATTTGATTGC
 3751 GAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC CGAGACAGAA
 3801 CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG
 3851 ATGCTCCACG CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT
 3901 TGATGGGTGT CTGGTCAGAG ACATCAAGAA ATAACGCCGG AACATTAGTG
 3951 CAGGCAGCTT CCACAGCAAT GGCATCCTGG TCATCCAGCG GATAGTTAAT
 4001 GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGCACC GCCGCTTTAC
 4051 AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC GCTGGCACCC
 4101 AGTTGATCGG CGCGAGATTT AATCGCCGCG ACAATTTGCG ACGGCGCGTG
 4151 CAGGGCCAGA CTGGAGGTGG CAACGCCAAT CAGCAACGAC TGTTTGCCCG
 4201 CCAGTTGTTG TGCCACGCGG TTGGGAATGT AATTCAGCTC CGCCATCGCC
 4251 GCTTCCACTT TTTCCCGCGT TTTCGCAGAA ACGTGGCTGG CCTGGTTCAC
 4301 CACGCGGGAA ACGGTCTGAT AAGAGACACC GGCATACTCT GCGACATCGT
 4351 ATAACGTTAC TGGTTTCACA TTCACCACCC TGAATTGACT CTCTTCCGGG
 4401 CGCTATCATG CCATACCGCG AAAGGTTTTG CGCCATTCGA TGGTGTCCGG
 4451 GATCTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT
 4501 AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCCG
 4551 GCATGCCGCC CTTTCGTCTT CAAGAATTAA TTCCCAATTC CCCAGGCATC
 4601 AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT
 4651 TGTTTGTCGG TGAACGCTCT CCTGAGTAGG ACAAATCCGC CGGGAGCGGA
 4701 TTTGAACGTT GCGAAGCAAC GGCCCGGAGG GTGGCGGGCA GGACGCCCGC
 4751 CATAAACTGC CAGGAATTAA TTCCCCAGGC ATCAAATAAA ACGAAAGGCT
 4801 CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC
 4851 TCTCCTGAGT AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC
 4901 AACGGCCCGG AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGAAT
 4951 TAATTCCCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC
 5001 CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA
 5051 ATCCGCCGGG AGCGGATTTG AACGTTGCGA AGCAACGGCC CGGAGGGTGG
 5101 CGGGCAGGAC GCCCGCCATA AACTGCCAGG AATTAATTCC CCAGGCATCA
 5151 AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT
 5201 GTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC GGGAGCGGAT
 5251 TTGAACGTTG CGAAGCAACG GCCCGGAGGG TGGCGGGCAG GACGCCCGCC
 5301 ATAAACTGCC AGGAATTAAT TCCCCAGGCA TCAAATAAAA CGAAAGGCTC
 5351 AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT
 5401 CTCCTGAGTA GGACAAATCC GCCGGGAGCG GATTTGAACG TTGCGAAGCA
 5451 ACGGCCCGGA GGGTGGCGGG CAGGACGCCC GCCATAAACT GCCAGGAATT
 5501 GGGGATCGGA ATTAATTCCC GGTTTAAACC GGGGATCTCG ATCCCGCGAA
 5551 ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC AATTCCCCTC
 5601 TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGGCTAGCA
 5651 AGTGGGTGAC TTTTATTTCT CTTCTCCTTC TCTTCAGCTC TGCTTATTCC
 5701 AGGGGTGTGT TTCGTCGAGA TAAACACAAG AGTGAGATTG CTCATCGGTT
 5751 TAAAGATTTG GGAGAAGAAC ATTTTAAAGG CCTGGTACTG ATTGCCTTTT
 5801 CTCAGTATCT CCAGCAGTGT CCATTTGATG AGCATGTAAA ATTAGTGAAC
 5851 GAACTAACTG AGTTTGCAAA AACATGTGTT GCTGATGAGT CCCATGCCGG
 5901 CTGTGAAAAG TCACTTCACA CTCTCTTTGG AGATGAATTG TGTAAAGTTG
 5951 CATCCCTTCG TGAAACCTAT GGTGACATGG CTGACTGCTG TGCGAAACAA
 6001 GAGCCTGAAA GAAATGAATG CTTCCTGAGC CACAAAGATG ATAGCCCAGA
 6051 CCTCCCTAAA TTGAAACCAG ACCCCAATAC TTTGTGTGAT GAGTTTAAGG
 6101 CAGATGAAAA GAAGTTTTGG GGAAAATACC TATACGAAAT TGCTAGAAGA
 6151 CATCCCTACT TTTATGCACC AGAACTCCTT TACTATGCTA ATAAATATAA
 6201 TGGAGTTTTT CAAGAATGCT GCCAAGCTGA AGATAAAGGT GCCTGCCTGC
 6251 TACCAAAGAT TGAAACTATG AGAGAAAAAG TACTGACTTC ATCTGCCAGA
 6301 CAGAGACTCA GGTGTGCCAG TATTCAAAAA TTTGGAGAAA GAGCTTTAAA
 6351 AGCATGGTCA GTAGCTCGCC TGAGCCAGAA ATTTCCCAAG GCTGAGTTTG
 6401 TAGAAGTTAC CAAGCTAGTG ACAGATCTCA CAAAAGTCCA CAAGGAATGC
 6451 TGCCATGGTG ACCTACTTGA ATGCGCAGAT GACAGGGCAG ATCTTGCCAA
 6501 GTACATATGT GATAATCAAG ATACAATCTC CAGTAAACTG AAGGAATGCT
 6551 GTGATAAGCC TTTGTTGGAA AAATCCCACT GCATTGCTGA GGTGGAAAAA
 6601 GATGCCATAC CTGAAAACCT GCCCCCATTA ACTGCTGACT TTGCTGAAGA
 6651 TAAGGATGTT TGCAAAAACT ATCAGGAAGC AAAAGATGCC TTCCTGGGCT
 6701 CGTTTTTGTA TGAATATTCA AGAAGGCATC CTGAATATGC TGTCTCAGTG
 6751 CTATTGAGAC TTGCCAAGGA ATATGAAGCC ACACTGGAGG AATGCTGTGC
 6801 CAAAGATGAT CAACATGCAT GCTATTCCAC AGTGTTTGAC AAACTTAAGC
 6851 ATCTTGTGGA TGAGCCACAG AATTTAATCA AACAAAACTG TGACCAATTC
 6901 GAAAAACTTG GAGAGTATGG ATTCCAAAAT GAGCTCATAG TTCGTTACAC
 6951 CAGGAAAGTA CCCCAAGTGT CAACTCCAAC TCTCGTGGAG GTTTCAAGAA
 7001 GCCTAGGAAA AGTGGGTACT AGGTGTTGTA CAAAGCCGGA ATCAGAAAGA
 7051 ATGCCCTGTG CTGAAGACTA TCTGAGCTTG ATCCTGAACC GGTTGTGCGT
 7101 GCTGCATGAG AAGACACCAG TGAGTGAAAA AGTCACCAAG TGCTGCACAG
 7151 AGTCATTGGT GAACAGACGG CCATGTTTCT CTGCTCTGAC ACCTGATGAA
 7201 ACATATGTAC CCAAAGCCTT TGATGAGAAA TTGTTCACCT TCCATGCAGA
 7251 TATATGCACA CTTCCCGATA CTGAGAAACA AATCAAGAAA CAAACTGCAC
 7301 TTGTTGAGCT GTTGAAACAC AAGCCCAAGG CAACAGAGGA ACAACTGAAA
 7351 ACCGTCATGG AGAATTTTGT GGCTTTTGTA GGCAAGTGCT GTGCAGCTGA
 7401 TGACAAAGAG GCCTGCTTTG CTGTGGAGGG TCCAAAACTT GTTGTTTCAA
 7451 CTCAAACAGC CTTAGCCTGC ATCACGGGAG ATGCACTAGT TGCCCTACCC
 7501 GAGGGCGAGT CGGTACGCAT CGCCGACATC GTGCCGGGTG CGCGGCCCAA
 7551 CAGTGACAAC GCCATCGACC TGAAAGTCCT TGACCGGCAT GGCAATCCCG
 7601 TGCTCGCCGA CCGGCTGTTC CACTCCGGCG AGCATCCGGT GTACACGGTG
 7651 CGTACGGTCG AAGGTCTGCG TGTGACGGGC ACCGCGAACC ACCCGTTGTT
 7701 GTGTTTGGTC GACGTCGCCG GGGTGCCGAC CCTGCTGTGG AAGCTGATCG
 7751 ACGAAATCAA GCCGGGCGAT TACGCGGTGA TTCAACGCAG CGCATTCAGC
 7801 GTCGACTGTG CAGGTTTTGC CCGCGGAAAA CCCGAATTTG CGCCCACAAC
 7851 CTACACAGTC GGCGTCCCTG GACTGGTGCG TTTCTTGGAA GCACACCACC
 7901 GAGACCCGGA CGCCCAAGCT ATCGCCGACG AGCTGACCGA CGGGCGGTTC
 7951 TACTACGCGA AAGTCGCCAG TGTCACCGAC GCCGGCGTGC AGCCGGTGTA
 8001 TAGCCTTCGT GTCGACACGG CAGACCACGC GTTTATCACG AACGGGTTCG
 8051 TCAGCCACGC TACTGGCCTC ACCGGTCTGA ACTCAGGCCT CACGACAAAT
 8101 CCTGGTGTAT CCGCTTGGCA GGTCAACACA GCTTATACTG CGGGACAATT
 8151 GGTCACATAT AACGGCAAGA CGTATAAATG TTTGCAGCCC CACACCTCCT
 8201 TGGCAGGATG GGAACCATCC AACGTTCCTG CCTTGTGGCA GCTTCAACAC
 8251 CACCATCATC ATCACCACCA CTGACTGCAG GAAGGGGATC CGGCTGCTAA
 8301 CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC
 8351 TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG
 8401 AAAGGAGGAA CTATATCCGG ATAACTACGT CAGGTGGCAC TTTTCGGGGA
 8451 AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT
 8501 GTATCC
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