PEG purification of PCR products

Overview

This is a simple and cost-effective protocol for purifying PCR products from primers and unused dNTPs. Purified products can be used in any downstream application, such as DNA sequencing.

To make PEG

• 3g PEG 8000
• 2.19g NaCl
• Bring up to 15ml with good water.
• Lasts for at least 30 days at 4C

Protocol

• Add 1:1 volume of PEG to PCR product, for example into the reaction tube.
• Vortex briefly.
• Warm at 37degrees for 15minutes.
• Microcentrifuge for 15minutes at max rpm.
• Pipette off supernatant.
• Wash pellet with exess 150ul 80% cold ethanol.
• Spin for 10minutes at at max rpm.
• Pipette off ethanol.
• [Optional] Wash with 95% ethanol.
• Dry down.
• Re-suspend in 10 to 25ul of good water. Generally, I re-suspend 8μL of a PCR product in 12μL water.