Paulsson:Pmalkus thoughts

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Improved response time

Estimate GFP-ssrA that can be degraded by ClpXP/ClpAP in molecules / min
Compare to reasonable detection limit in FACS

Use pBAD UBP1 to modulate degradation rate

How to measure degradation rate below detection level? - can't be fluorescence!!!

Western or pulse/chase IP


Do the same for PEST / proteasome in S.cerevisiae


Critical parameters for plasmid counting

Doubling time
Plasmid loss rate (when using ts plasmid)

FP reporter

synthesis rate
degradation rate
maturation rate

How do these vary with temperature, growth media...

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