Pelling:Protocols/DMEM

From OpenWetWare

Jump to: navigation, search
Bishop.jpg


Basic DMEM Media Formulation (10% FBS, 1% P/S) - Complete

  • Thaw 5ml P/S and 50ml FBS in 37°C water bath.
  • Obtain a new 500ml bottle of DMEM (with l-Glutamine) from the fridge.
  • Remove 55ml from DMEM using a 25ml pipette into a waste beaker.
  • Using the same tip, dispense 50 ml of FBS in the DMEM.
  • Use a clean 5ml pipette to withdraw 5ml of P/S and dispense into the DMEM.
  • Final formulation: 50ml FBS + 5ml P/S + 445ml DMEM
Personal tools