Post-Processing of Oligo Arrays on Corning Ultra GAPS slides

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Image:Hum_array2.gif

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Post-processing slides

  1. Using a diamond scribe, etch the non-printed side of the array to denote the boundaries of the printed area
  2. UV cross-link printed DNA onto glass substrate with 65mJ of energy in a Stratalinker (650 x 100uJ) and transfer arrays on a slide rack
  3. Prehybridization in 5x SSC, 0.1 mg/ml BSA, 0.1% SDS at 42°C for 60 Min. (300 ml MQ H2O, 100 ml filtered 20 x SSC, 4 ml 10mg/ml BSA stock solution (filtered), 4 ml 10% SDS, prewarm in 42°C waterbath for 60 min)
  4. Rinse in arrays in 0.1 x SSC for 5 min at RT
  5. Repeat step 4
  6. Transfer arrays to MQ water and incubate at RT for 30 sec.
  7. Spin dry arrays in a table to centrifuge at 500 rpm for 5 min.


NOTES:

  • Use arrays the same day that they are postprocessed
  • USE FOR CORNING ULTRA GAPS SLIDES ONLY, DO NOT USE ON POLYLYSINE COATED SURFACES



Martin Buess

DRAFT VERSION 01.17.06

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