Prince:UPLC

From OpenWetWare

Jump to: navigation, search

Image:Prince lab logo.png

Home        Lab Members         Research         Publications         Internal         Mass Spec         Contact        


Common Supplies

Name Description Company Catalog# Price($) Notes
Fused Silica Sleeves adapts from 360um to 1/32" OD VICI C-NL.35L 5 for $15
PicoFrit Column 100mm long, 360um OD, 75um ID, 15um tip, packed with 5um Biobasic C18 Thermo PFC7515-BI-10-3PK  ?? These came in the PepFinder Kit (we're not using these right now)
Fused Silica (25um ID; 360um OD) general purpose fused silica VICI TFS36025 $20/meter
Fused Silica (20um ID; 360um OD) ultra small (ID) fused silica Upchurch Scientific, Inc. FS-120 42.40 / 2meters
PEEKsil fused silica inside, PEEK outside SGE, Inc. [varies] [varies]
Uncoated Silica Tips Uncut silica spray tips (we can cut with cutter) Scientific Instruments Services [New Objective makes the tips] FS360-20-10-N-20 $252.00/20
Precut Silica Tips for ThermoFinnigan Nanospray 2 The spray tips [5 in package] Scientific Instruments Services [New Objective makes the tips] FS360-25-10-N-5-CT $135/5; $500/20
Ferrule, Fingertight 1/32, for 10-32 ports ferrules Upchurch Scientific, Inc. F-113 7.65 ea
Ferrule, Fingertight 360um, for 10-32 ports ferrules Upchurch Scientific, Inc. F-151 11.70 ea
Nut, WINGED, Fingertight 1/16, for 10-32 ports winged nut Upchurch Scientific, Inc. F-300-01 2.70 ea standard nut for the system
Nut, LONG, Fingertight 1/16, for 10-32 ports long nut Upchurch Scientific, Inc. F-330-01 2.70 ea standard nut for the system
Nut, SHORT, Fingertight 1/16, for 10-32 ports short nut Upchurch Scientific, Inc. F-331-01 2.70 ea standard nut for the system


Flow Rates

Table 2-3. Effective flow rate range SilicaTip emitters (from Finnigan Nanospray Ion Source Operator’s Manual 97055-97025 Revision A, October 2004)

Emitter ID (um) Flow rate (nL/min)
5 20 to 100
8 100 to 300
10 100 to 400
15 150 to 400
30 300 to 1000


Table 9-1. LC/NSI/MS Parameters for Finnigan LTQ or LCQ Series mass spectrometers (Finnigan™ Nanospray Ion Source Operator’s Manual 97055-97025 Revision A October 2004)

Column Size Flow Rate Tip ID Emitter Position Capillary Temperature
50 μm <100 to 300 nL/min 5 to 15 μm approx. 1 mm 100 to 180 °C
75 μm 150 to 800 nL/min 10 to 20 μm 1 to 2 mm 130 to 200 °C
180 μm 0.3 to 1.0 μL/min 20 to 30 μm 2 to 3 mm 150 to 250 °C
320 μm 1 to 5 μL/min 30 to 75 μm 3 to 5 mm 150 to 250 °C

This is from the Thermo Chromatography Catalogue 2006-2007 (pg. 177).

ColumnID (mm) Flow Rate Range (uL/min) Optimum Flow Rate (ul/min) Recommended Injection Vol. (ul) API Source
0.32 4 - 10 5 0.15 Micro-ESI
0.18 1 - 3 2 0.05 Micro-ESI
0.1 0.4 - 1 0.5 0.015 Nanospray
0.075 0.2 - 0.5 0.3 0.01 Nanospray
Personal tools