- pick half of an isolated colony with a toothpick of pipet tip and inoculate 25 µl TE; store the plate parafilmed at 4ºC.
- boil for 5 min.
- spin down at 13k for 1 min.
- use 2 µl as template for subsequent pcr.
- run out on a gel with respective control.
- pick other half of the positive clones and inoculate liquid o/n culture for miniprep.