Reagents for SPR

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reagentspurposeMrhandling instructions
1-ethyl-3-(3-dimethyl

aminopropyl) carbodiimide (EDC)
ethanolamine

amine coupling 191.70 EDC stock solution 0.4M

resuspend fresh ultrapure reagent in milli-Q water
aliquote into 200μL portions directly into vials to be used on the instrument
freeze the aliquotes and store at -20 for no more then 2 months
if you buy EDC from Biacore - dissolve 0.75 g portion supplied in 10 mL water

N-hydroxysuccinimide (NHS)
amine coupling 115.09 NHS stock solution 0.1M

prepare and store as desribed for EDC
Biacore supplied 115 mg portion should be dissolved in 10 mL water

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