Registry/Measurement kit/Notebook/2007-11-9

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--Adam J. Rubin 16:20, 12 November 2007 (CST) Diluted 1:1000 (5ul in 5 ml M9 media) in the morning after overnight growth. Pre-warmed the M9 media before to counter temperature shock.

Noticed a difference in color. After 4.5 hours tubes 4 and 14 were noticeably more clear than all others. Made plate per standard procedure. [1]

Plate layout:

1. I20244 w/ J23116 (w) (GFP , E/S) x2 x3 9. I20245 w/ J23116 (w) (GFP, E/X) x2 x3
2. I20244 w/ R0040 (s) (GFP , E/S) x2 x3 10. I20245 w/ R0040 (s) (GFP, E/X) x2 x3
3. I20246 w/ J23116 (w) (RFP, E/S) x2 x3 11. I20247 w/ J23116 (w) (RFP, E/X) x2 x3
4. I20246 w/ R0040 (s) (RFP, E/S) x2 x3 12. I20247 w/ R0040 (s) (RFP, E/X) x2 x3
5. I20248 w/ B0031 (w) (GFP, E/X) x2 x3 13. I20249 w/ B0031 (w) (GFP, S/X) x2 x3
6. I20248 w/ B0032 (s) (GFP, E/X) x2 x3 14. I20251 w/ B0032 (s) (RFP, S/X) x2 x3
7. I20250 w/ B0031 (w) (RFP, E/X) x2 x3 15. blank M9 media x2 x3
8. I20250 w/ B0032 (s) (RFP, E/X) x2 x3 16. empty TOP10 cells x2 x3
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