Registry/Measurement kit/Notebook/2007-7-30

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To Do

  • See about making competent DB3.1 cells
  • Test the top10 stock with a + control (pUC18)
  • See what we can do with BB'd E0240 & I2057

Promoter Testers

  • Should pick some promoters to drop in.
  • R0040 + a strong promoter + a weak promoter
  • Using <bbpart>R0040</bbpart> and two of Anderson's promoters: <bbpart>J23116</bbpart> (weak) and <bbpart>J23100</bbpart> (strong)
  • Using Joey's transformation of the promoters.


  • Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR)
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