- Got growth on:
- 3K3 (from both sequential and double digests)
- 1AK3 (2 colonies)
- Will colony PCR
- Gel of I2055 (with promoter) looks ok, will sequence to make sure.
- PCR cleaned I2055
- Double M/N digest in buffer 2
- I2056 doesn't seem to grow in o.n. amp/tet culture...trying with just tet.
- Ligating T9002 into 1AK3