Registry/Measurement kit/Previous versions

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The Measurement Kit consists of Promoter Testers, RBS Testers, and an Inverter/Terminator Tester. All Devices are on the 3K3 plasmid. Also, they are being built in both GFP and RFP versions. (GFP versions shown below)


Promoter Tester

E0240 Version 1 Design
E0240 Version 1 Design

The Promoter Tester using GFP is based on <BBpart> E0240</BBpart> and using RFP is based on <BBpart> I2057</BBpart>. Both come in two versions.

  • Version 1 (E/S)
    • ___E X CCDB S___RBS GFP T
    • Cut promoter E/S and insert
    • Can take part back out if necessary
  • Version 2
    • ___E CCDB X___E0240
    • Cut Promoter E/S to insert
    • Forms mixed site so can't remove, real conditions
  • GFP Promoter Tester Version 1 E/S <BBpart> I20244</BBpart>
  • GFP Promoter Tester Version 2 E/X <BBpart> I20245</BBpart>
  • RFP Promoter Tester Version 1 E/S <BBpart> I20246</BBpart>
  • RFP Promoter Tester Version 2 E/X <BBpart> I20247</BBpart>

RBS Tester

I2055  Design
I2055 Design

The RBS Tester using GFP is based on <BBpart> I2055</BBpart> and using RFP is based on <BBpart> I2056</BBpart>. Both come in two versions.

  • Versions 1 (E/X)
    • __R0040___E CCDB X____GFP T
    • To insert RBS cut E/S, forms a mixed Spe/Xba site
    • Has too big spacing (full BB prefix) b/t promoter and RBS
    • Easier for testers to use
  • Version 2 (S/X)
    • __R0040___S CCDB X____GFP T
    • Will get 2 spe/xba mixed sites, can't get RBS out
    • Cut RBS X/S, ligate w/ Spe1 and Xba1 restriction enzymes to ensure proper orientation
    • Real condition spacing
  • GFP RBS Tester Version 1 E/X <BBpart> I20248</BBpart>
  • GFP RBS Tester Version 2 S/X <BBpart> I20249</BBpart>
  • RFP RBS Tester Version 1 E/X <BBpart> I20250</BBpart>
  • RFP RBS Tester Version 2 S/X <BBpart> I20251</BBpart>

Inverter/Terminator Tester

T9002 Design
T9002 Design
  • Based on <BBpart> T9002</BBpart>
  • F2620________E X P1010 S P________E0040
  • Contains a full BB site to allow construction within the device


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