Registry of standard biological parts/PCR-based assembly

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Demo

Going to get a better feel for this by doing a few biobrick constructions using PCR-assembly

  1. <bbpart>J04500</bbpart>+<bbpart>I13501</bbpart> = <bbpart>J04450</bbpart>
  2. <bbpart>R0010</bbpart>+<bbpart>I13504</bbpart> = <bbpart>J04430</bbpart>
  1. <bbpart>F2620</bbpart>+<bbpart>P1010</bbpart>+<bbpart>E0240</bbpart>
    • will try this one as a 3-way

UDG

Can use UDG (NEB USER enzyme) to replace all restriction digests and could also eliminate secondary PCRs also. You would just need to design your oligos to contain a U in the right location, assuming that there'll be a T somewhere in the vacinity of the ends of the parts you are trying to combine.

In-Fusion

References

Error fetching PMID 2357375:
Error fetching PMID 2748349:
Error fetching PMID 9591118:
Error fetching PMID 12139251:
Error fetching PMID 17389646:
Error fetching PMID 12235397:
Error fetching PMID 14739232:
Error fetching PMID 15780712:
Error fetching PMID 17158165:
Error fetching PMID 7950389:
  1. Error fetching PMID 15780712: [Lu-2005]
  2. Error fetching PMID 17158165: [Evans-2007]
  3. Error fetching PMID 2357375: [Pease-1990]
  4. Error fetching PMID 2748349: [Fried-1989]
  5. Error fetching PMID 9591118: [Karrenman-1998]
  6. Error fetching PMID 12139251: [Burnap-2002]
  7. Error fetching PMID 17389646: [Halkier-2007]
  8. Error fetching PMID 12235397: [Jarrell-2002]
  9. Error fetching PMID 14739232: [ladisch-2004]
  10. Error fetching PMID 7950389: [Traktman-1994]
All Medline abstracts: PubMed HubMed
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