Registry of standard biological parts/PCR-based assembly

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Contents

Demo

Going to get a better feel for this by doing a few biobrick constructions using PCR-assembly

  1. <bbpart>J04500</bbpart>+<bbpart>I13501</bbpart> = <bbpart>J04450</bbpart>
  2. <bbpart>R0010</bbpart>+<bbpart>I13504</bbpart> = <bbpart>J04430</bbpart>
  1. <bbpart>F2620</bbpart>+<bbpart>P1010</bbpart>+<bbpart>E0240</bbpart>
    • will try this one as a 3-way

UDG

Can use UDG (NEB USER enzyme) to replace all restriction digests and could also eliminate secondary PCRs also. You would just need to design your oligos to contain a U in the right location, assuming that there'll be a T somewhere in the vacinity of the ends of the parts you are trying to combine.

In-Fusion

References

  1. Lu Q. . pmid:15780712. PubMed HubMed [Lu-2005]
  2. Hamilton MD, Nuara AA, Gammon DB, Buller RM, and Evans DH. . pmid:17158165. PubMed HubMed [Evans-2007]
  3. Horton RM, Cai ZL, Ho SN, and Pease LR. . pmid:2357375. PubMed HubMed [Pease-1990]
  4. Yon J and Fried M. . pmid:2748349. PubMed HubMed [Fried-1989]
  5. Karreman C. . pmid:9591118. PubMed HubMed [Karrenman-1998]
  6. Wang HL, Postier BL, and Burnap RL. . pmid:12139251. PubMed HubMed [Burnap-2002]
  7. Geu-Flores F, Nour-Eldin HH, Nielsen MT, and Halkier BA. . pmid:17389646. PubMed HubMed [Halkier-2007]
  8. Donahue WF, Turczyk BM, and Jarrell KA. . pmid:12235397. PubMed HubMed [Jarrell-2002]
  9. Shevchuk NA, Bryksin AV, Nusinovich YA, Cabello FC, Sutherland M, and Ladisch S. . pmid:14739232. PubMed HubMed [ladisch-2004]
  10. McDonald WF and Traktman P. . pmid:7950389. PubMed HubMed [Traktman-1994]
All Medline abstracts: PubMed HubMed
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