Restriction Digest with HaeIII

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  • Fabric Name BsuRI
  • Restictive Enzyme: BsuRI (HaeIII) store at -20°C
  • Plasmid: pBSKS concentration 4μg/μL
  • 20μL final volume
  • add restictive enzyme to the mixture last
  • after everything is mixed, incubate at 37°C for at least one hour


  • 2μL buffer R
  • 3μL plasmid
  • 2μL enzyme
  • 13μL D H2O


  • 2μL buffer R
  • 3μL plasmid
  • 0μL enzyme (no enzyme)
  • 15μL DH2O

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