Restriction Digest with HaeIII

From OpenWetWare

Jump to: navigation, search

Notes

  • Fabric Name BsuRI
  • Restictive Enzyme: BsuRI (HaeIII) store at -20°C
  • Plasmid: pBSKS concentration 4μg/μL
  • 20μL final volume
  • add restictive enzyme to the mixture last
  • after everything is mixed, incubate at 37°C for at least one hour

Experimental

  • 2μL buffer R
  • 3μL plasmid
  • 2μL enzyme
  • 13μL D H2O

Control

  • 2μL buffer R
  • 3μL plasmid
  • 0μL enzyme (no enzyme)
  • 15μL DH2O


Back to Protocols

Personal tools