RiPS
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Ribosomes Per Second
A standard unit to measure translational activity of an mRNA molecule.
RiPS per mRNA are equivalent to the rate of the limiting step in ribosome initiation and elongation.
| Study | Value | Note |
|---|---|---|
| Kennell et al. [1] | 0.3 Protein mRNA-1 s-1 | Measured for the lac operon in E. coli, specifically, the lacZ gene |
| 0.06 Protein mRNA-1 s-1 | Measured for the lac operon in E. coli, specifically, the lacA gene | |
| De Smit et al. [2] | 0.5 Protein mRNA-1 s-1 | Assumed this value based on Tomsic et al. [3] |
| Jennifer Braff & Caitlin Conboy - Soup to nuts | 0.039-0.55 Protein mRNA-1 s-1 | Measured using BBa_I7107 and BBa_I7109 on pSB3K3 and pSB4A3. |
References
- Kennell D and Riezman H. Transcription and translation initiation frequencies of the Escherichia coli lac operon. J Mol Biol. 1977 Jul;114(1):1-21. DOI:10.1016/0022-2836(77)90279-0 |
- de Smit MH and van Duin J. Translational standby sites: how ribosomes may deal with the rapid folding kinetics of mRNA. J Mol Biol. 2003 Aug 22;331(4):737-43. DOI:10.1016/s0022-2836(03)00809-x |
- Tomsic J, Vitali LA, Daviter T, Savelsbergh A, Spurio R, Striebeck P, Wintermeyer W, Rodnina MV, and Gualerzi CO. Late events of translation initiation in bacteria: a kinetic analysis. EMBO J. 2000 May 2;19(9):2127-36. DOI:10.1093/emboj/19.9.2127 |
