RobWardenWNB:M3D6

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Expression Engineering, Day 6, 4/25/07

Microarray Analysis

Red Signal
NY389
Green Signal
FY2068

Notes

  1. Copied only GeneName, SystematicName, Description, gMeanSignal, rMeanSignal gMedianSignal, rMedianSignal, gBGMeanSignal, rBGMeanSignal, gBGMedianSignal, rBGMedianSignal onto Analysis worksheet
  2. Deleted Unused Controls
    • NegativeControls
    • EQCs
    • A_##_P##
  3. Corrected for Background
    • BGCor = MedianSignal - BGMedianSignal
    • Gave LOTS of netative numbers for green
  4. Normalized Data
    • rNorm = rBGCor * avg(gBGCor) / avg(rBGCor)
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