SBB09 Construct40
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Construction of upaG (long) basic part
PCR js001F/js002R on Escherichia coli CFT073 (4228 bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, 2974+910, L)
Product is pBca9495KC-M10054 {<upaG_long!}
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js001F Forward biobricking of upaG (long) CCATAgaattcATGagatctcgcaaaattgttaatatggctgctg
js002R Reverse biobricking of upaG (long) CTGATggatccCCACtgaataccggcaccga
JCA: You designed a {<part>}, you need a stop codon in reverse oligo. See revision:
Construction of upaG (long) basic part
PCR js001F/js002R on Escherichia coli CFT073 (4228 bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, 2974+910, L)
Product is pBca9495KC-M10054 {<upaG_long!}
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js001F Forward biobricking of upaG (long) CCATAgaattcATGagatctcgcaaaattgttaatatggctgctg
js002R Reverse biobricking of upaG (long) CTGATggatccTTACCACtgaataccggcac
