# SBB12 gels

### From OpenWetWare

SynBioBootcamp Homepage

How to Load, Run, and Upload Gels

## Ladder

Unless otherwise noted, we will be using the NEB 2-log ladder. NEB site

## Example

### JCAnderson 14:40, 17 February 2011 (EST)

021711-AnalGel1 (just an example)

This one used 1kb plus.

## Gels: Feb 17

### MW ladder used in these gels

### Au Duong 13:35, 17 February 2012 (EST)

### Zachary Russ 13:43, 17 February 2012 (EST)

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

JW A | JW B | MM | empty | MW ladder | JS | DC A | DC B | empty | empty |

## Gels: Feb 21

### Tina Nie 13:56, 21 February 2012 (EST)

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

Au PCA2 | TN sbb1213 | DC A | DC B | 2-log ladder | JS | MM | JW | RO TP | RO CT |

### Tina Nie 14:10, 21 February 2012 (EST)

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

LZ AAAA PCA2 | sbb1224 | sbb1203 | 2-log ladder | AJ pca2 | MD | JK caff-PCA2 | AJ A | AJ B | PR PCR1 |

## Gels: Feb 23

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

MM | PR-PCA | JS | Tar dig | 2-log ladder | Jkotker LZ (1204) | MD PhoA | sbb1224 | MD LZ | MH PCR A+B |

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

GF 438 | GF 2801 | empty | JKotker Caff (1223) | empty | 2-log ladder | PR-PCR | Karen PCRproduct | empty | Au SOE digest |

## Gels: Feb 24

### Gel 1

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

MH PCRPDT 1218 | MH PCRDIG 1218 | empty | DC SB 1221 | Ladder | empty | empty | empty | GF PCA | RO SOE3 1231 |

## Gels: Feb 28

### Au Duong 14:19, 28 February 2012 (EST)

### Robert D O'Dowd 14:19, 28 February 2012 (EST)

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

empty | AJ A+B pcrpdt | empty | RD 1231 dig | empty | 2-log ladder | empty | empty | empty | empty |

## Gels: Mar 1

### Class Gel #1

### Class Gel #2

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

Karin 1206 | 1224(1) | 1224(2) | 1224(3) | 1203(1) | ladder | AJ | RO TM PhoI | RO CITM | Bad Load |

## Gels:March 1 (Gel 3)

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

entry one | two | 1217 | four | ladder | Lz-AAAA1(1) | Lz-AAAA1(2 | eight | nine | ten |

## Gels: Mar 2

### Gel 1

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

Au SOE3 | AJ A+B | empty | DC A+B | 2log | empty | empty | empty | JW A2 | 2log |

### Gel 2

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

AJ A+B | AJ A+B | MM 1232-1 | MM 1232-2 | MM 1215-2 | RO MP 1214 | Ladder | MDP2 | MDP1 | MDL2 |

### Gel 3

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

KA miniprep1 1206 | KA miniprep2 1206 | KA miniprep3 1206 | HH Tar 1 | 2log ladder | HH Tar 2 | HH LZ 1 | HH LZ 2 | MH 1207-1 | MH 1207-2 |

## Gels: Mar 6

### Gel 1

### Class Gel 2

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

AJ A+B | JS 1201-1 | JS 1201-2 | JS 1226-1 | JS 1226-2 | Ladder | PR 02-1 | PR 02-2 | PR 30-1 | JK 1233-1 |

### Gel 3

### Class Gel 4

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

MM 1232-1 | MM 1232-2 | MM 1215-2 | DC 1221 A+ B Pur | Ladder | JK 1233-2 | JK 1204-1 | JK 1204-2 | PR 30-2 | PR 30-3 |

### Class Gel 5

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

PR 30-4 | PR 02-1 | PR 02-2 | PR 02-3 | PR 02-4 | Ladder | JK 1223-1 | AJ 12205-1 | AJ 12205-2 | AJ 12205-3 |

## Gels: Mar 8

### Class Gel 1

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

Ladder 2μL | GF PCA | Test Ladder 1:5 10μL | JW A4 | Ladder 3μL | LZ AAAA-1 | JW A4 | 1224 M1 | 1224 M2 | MH A+B pcrpdt |

### Class Gel 2

### Class Gel 3

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

AJ Digest 1 | AJ Digest 2 | AJ Digest 3 | AJ Digest 4 | Test Ladder 10μL | TN sbb1227 3 | TN sbb1227 4 | JK 1204-1 | AJ A+B Digest (cut) | Test Ladder 10μL |

## Gels: Mar 13

### Class Gel 1

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

Karen A sbb1217 mp1 | Karen A sbb1217 mp2 | Karen A sbb1217 mp3 | Karen A sbb1217 mp4 | Lader | sbb1224(1) | sbb1224(2) | AJ A+B | Karen A sbb1217 mp3 | Ladder |

### Class Gel 2

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

DC sbb1221 A+DMSO | DC sbb1221 A | DC sbb1221 B+DMSO | DC sbb1221 B | Lader | MH 1218 dig | JV A5 | empty | empty | empty |

### Class Gel 3

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

RO 1214 Dig | RO 1231 Dig | AJ A+B PCR digest | **** | Lader | KA | KA | KA | KA | ladder |

## Gels: Mar 15

### Class Gel 1

### Class Gel #2

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

sbb1203(1) | sbb1203(2) | sbb1203(3) | sbb1203(4) | ladder | sbb1224(1) | sbb1224(1 | sbb1224(2) | sbb1224(3) | sbb1224(4) |

Karen D. Alvarez 15:34, 15 March 2012 (EDT)

### Class Gel #3

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

sbb1217(1) | sbb1217(2) | sbb1217(3) | sbb1217(4) | ladder | ladder | -- | -- | -- | -- |

## Gels: March 20

### Class Gel 1

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

none | MM 1 | MM 2 | MM 3 | MM 4 | ladder | DC A+B | AJ gel purify | GF pcrpdt | none |

### Class Gel 2

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

Ladder | empty | JW BD | empty | Ladder | empty | JW A6-B | Ladder | empty | JW A6-B |

### Class Gel 3

## Gels: March 22

### Class Gel 1

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

none | RO 1214 1 | RO 1214 2 | RO 1214 3 | RO 1214 4 | 2-log | AJ digest | DC A+B | JW A10 | none |

### Class Gel 2

Lanes:

1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|

none | none | none | none | 2-log | DC A+B Digest | none | none | none | none |