SEED/2011/Schedule

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Overview of the main lab project

Class Date Agenda Homework Lab Protocol
Day 1 Feb. 5
  • Introductions
  • Molecular Biology Review
  • Units Review
  • Homework #1: doc pdf
    • read comic
    • iGEM Questions
    • Ideas (3-5) for what you want to engineer biology to do
    • Training lab questions
    • Data Analysis
Lab Day #1: Serial Dilutions doc
Day 2 Feb. 12 SynBio Overview, sterile technique, colony streaking, antibiotic resistance Lab Day #2: Plate Streaking pdf doc
Day 3 Feb. 26 cloning, PCR
  • Lab Day #3: Cloning and PCR docx pdf
Day 4 Mar. 5 miniprep, restriction digestion
  • Lab Day #4: Miniprep and Restriction Digestion docx pdf
Day 5 Mar. 12 gel electrophoresis
  • Lab Day #5: Gel Electrophoresis docx pdf
Day 6 Mar. 26 ligation, transformation
  • Pre-Lab #6: none
  • Midterm: docx pdf
Lab Day #6: Ligation and Transformation docx pdf
Day 7 Apr. 2 prep for sequencing, glycerol Lab Day #7: docx pdf
Day 8 Apr. 9 analyze sequencing, smell test, final projects
  • Homework #8:
Lab Day #8:
Day 9 Apr. 23 FINAL, final projects
  • Homework #9:
Lab Day #9:
Day 10 April 30 MIT 150!
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