SOB Media

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DH5α Competent Cells

SOB Media
Reagent/Final Vol 400 ml 800 ml 1600 ml
Tryptone 8 g 16 g 32 g
Yeast 2 g 4 g 8 g
NaCl 0.2 g 0.4 g 0.8 g
0.25 M KCl (add after dissolving the foregoing) 4 ml 8 ml 16 ml

pH 7.0 with 5 N NaOH. Autoclave. Add 2 mls filter sterilized 2M MgCl2 to each 400 ml.

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