=1st PCR product purification by PCR purification kit
- 5 volumes of buffer PB added to volume of the PCR reaction and mixed
- A QIAquick column is plaed ina provided 2 ml collection tube
- To bind DNA, the sample is applied to the QIAquick column and centrifuged for 45 sec. Flow trough is discarded and QIAquick column is placed back into the same tube.
- To wash 0,75 ml buffer PE is added to the QIAquick column and centrifuged for 45 sec .Flow through is discaded and QIAquick column is placed back into the same tube.
- The column is centrifuged in a 2ml collection tube for 1min
- Each QIA quick column is placed in a clean 1.5 ml microcentrifuge tube.
- The elute DNA for increased DNA concentration 30 ul EB buffer added to the center of the QIAquick membrane let the column stand for 1 min and than centrifuged.
- 1-11 - gfp oligos : 100,3 ng/uL
- 12-22 gfp oligos : 89,7 ng/uL