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SDS-PAGE on peanut protein from Mt Sinai
Ran SDS-PAGE with Bert to gauge protein purity/concentration of Ara h 2, Ara h 3, and Ara h 6 stock from Mt Sinai, each with a stated concentration of 3.4 mg/ml. Ran nanodrop to determine approximate protein concentrations:
Made loading solutions for each protein as follows:
Loaded the gel w/ 1 µg and 5 µg for each protein.
According to Bert, band sizes, when matched to bands of known protein concentration (from his past gels), reflected nanodrop concentrations fairly well.