Shreffler:Notebook/Basophil Imaging

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Contents

Overview

Nanotubles

  • Reproduce
  • Structure
    • DNase
    • Actin
  • Time lapse
  • Function
    • depolarization (Ca flux)
    • intercellular transport
  • Activation dependence

Team

  • Alex Praslick
  • Matthew Gross
  • Madhan Masilimani
  • Wayne Shreffler

Meeting Notes

23 June

Alex and I met to discuss priorities for the summer.

  • Reproducibility of activation-dependent putative nanotubes
  • Characterization
    • Physical dimensions
    • Polarization
    • Actin: phalloidin
    • DNA: intercalating dyes, DNase treatment -- consult reference [1]
  • time lapse experiments

To Do:

  • order phalloidin
  • order mAbs
  • order new DAPI
  • review and decide on protocols for phalloidin staining as well as DNA staining/ digestion

Future Projects

Ag uptake

  • IgE-dependence
  • Traffic
  • Interaction with T cells

Activation-induced morphological changes

  • correlation of changes with flow
  • non-releaser phenotype
  • polarization
  • phosphorylation events
  • impact on intercellular activation?

References

  1. Yousefi S, Gold JA, Andina N, Lee JJ, Kelly AM, Kozlowski E, Schmid I, Straumann A, Reichenbach J, Gleich GJ, and Simon HU. . pmid:18690244. PubMed HubMed [Yousefi_2008]
  2. Denzel A, Maus UA, Rodriguez Gomez M, Moll C, Niedermeier M, Winter C, Maus R, Hollingshead S, Briles DE, Kunz-Schughart LA, Talke Y, and Mack M. . pmid:18516038. PubMed HubMed [Denzel_2008]
  3. Sanbar SS. . pmid:894382. PubMed HubMed [Dvorak_2000]
  4. Dvorak AM. . pmid:15970657. PubMed HubMed [Dvorak_2005]
  5. Galli SJ and Franco CB. . pmid:18400194. PubMed HubMed [Galli_2008]
  6. Hennersdorf F, Florian S, Jakob A, Baumgärtner K, Sonneck K, Nordheim A, Biedermann T, Valent P, and Bühring HJ. . pmid:15916720. PubMed HubMed [Hennersdorf_2005]
  7. Mack M, Schneider MA, Moll C, Cihak J, Brühl H, Ellwart JW, Hogarth MP, Stangassinger M, and Schlöndorff D. . pmid:15634893. PubMed HubMed [Mack_2005]
  8. Min B, Prout M, Hu-Li J, Zhu J, Jankovic D, Morgan ES, Urban JF Jr, Dvorak AM, Finkelman FD, LeGros G, and Paul WE. . pmid:15314076. PubMed HubMed [Min_2004]
  9. Shin JS, Shelburne CP, Jin C, LeFurgey EA, and Abraham SN. . pmid:17056503. PubMed HubMed [Shin_2006]
  10. Sokol CL, Barton GM, Farr AG, and Medzhitov R. . pmid:18300366. PubMed HubMed [Sokol_2008]
  11. Wu M, Baumgart T, Hammond S, Holowka D, and Baird B. . pmid:17698921. PubMed HubMed [Wu_2007]
  12. Yousefi S, Mihalache C, Kozlowski E, Schmid I, and Simon HU. . pmid:19609275. PubMed HubMed [Yousefi_2009]
All Medline abstracts: PubMed HubMed
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