Shreffler:Notebook/Staff Meetings/2010/05/19

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*Wayne G. Shreffler 15:47, 19 May 2010 (EDT):

Agenda Items

  • Protein fractionation
    • good recovery
    • ordered SDS-PAGE supplies
    • transcriptional assay as soon as Friday
    • Neet to email Steve about names of facilities for analysis and his insight having reviewed Kaye's protocol
  • Co-culture CBA data:
    • low levels of IL-5, IL-13 -- high levels of gamma from this donor. In general more cytokine from SEB driven cultures -- in contrast to mRNA
  • bio-significance: need to re-prime the foreskin pump
    Bert will first compare new lot of stemcell media and finish up comparing fractions
  • R / Bioconductor course next week
    Bert will check prereq's for course


Facilities:

  • Freezer -- this week?




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