Shreffler:Notebook/Staff Meetings/2010/08/03

ShreffLab Staff Meetings

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*Wayne G. Shreffler 10:49, 3 August 2010 (EDT): Agenda Items EoE EDN ELISAs Sarita has list from Marcella and will order a kit or two to evaluate Component proposal -- WS still needs to work on Basophil Review data from last week (Sarita, Alex sens #7,8) Sarita's experiment low baso counts, but good Ag response evidence of pre-existing Derp2 sensitization; however, clear evidence of stronger degran with 50:50 sens than 99:1 or 1:99 Next experiments Repeat with buffy's this week, consider lengthening stripping time? Caitlin paper? -- Bert has looked? no discussion today Allergen Adjuvant Review outcome of meeting w/ S. Carr Willing to perform digest/ MS and see Review most recent PCR data on dose response to fraction 3 strong evidence of correlation with prominent band on dilution experiment -- very nice response, much stronger in Fr3 than whole CPE as well F/U with Carr Bert will take gels over THIS week Forski's? decent number of CD45+DR+CD1a+, but no evidence of Aledflour enhancement; staining/ flow not optimal PM Baso nothing new JAX quote for peptide arrays WS has to do train Stephanie for project role U19 Discuss involvement with Bert Other Discussion Isabelle will see bakery owner on Thu; working on dietary guideline doc, will meet w/ her tomorrow morning Things To Do Investigate EDN assay options/ request samples w/ standards (Sarita) Write up component proposal (Marcella and Wayne) obtain quote for peptide arrays send Bert U19 slides send Isabelle Dowling the dining services director name/ contact