Small molecule binding by SPR

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Drug-like small organic molecules often do not dissolve well in water. Small concentration (<10%) of DMSO is used then to improve drug solubility for SPR analysis. A common technique is to add DMSO solution of drug to the aqueous buffer [1].

Dielectric properties of DMSO and water are significantly different. As a result, even when the test solution contains no analyte there can be large offset in RU between the reference and test cells. Karlsson et al [1] have suggested a calibration protocol that eliminates this offset.

  1. Frostell-Karlsson A, Remaeus A, Roos H, Andersson K, Borg P, Hämäläinen M, and Karlsson R. . pmid:10821711. PubMed HubMed [karlsson1]
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