Smolke:Oligos

From OpenWetWare

Overview

• resuspension of Oligos from IDT

Materials

• TE (10mM Tris pH 8.0, 1 mM EDTA)

Procedure

1. add 10uL TE for every nM DNA (makes 10x stock at 100uM)
2. dilute 1:10 to make 10uM stock (for PCR etc)

Notes

• 10x stock should be dethawed with minimal frequency

References

• IDT FAQ

Contact

• Kelsic