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- 16:33, 31 March 2012 (hist) (diff) N McClean:LFM Proline (New page: ==Overview== Standard LFM Media but with Proline as the nitrogen source. As always, leave out glucose if the carbon source is variable and use amino acid mix as needed. ==Final Desired...) (top)
- 11:43, 30 September 2011 (hist) (diff) McClean:Links
- 00:38, 15 August 2011 (hist) (diff) McClean:Protocols (→Yeast)
- 00:38, 15 August 2011 (hist) (diff) McClean:Protocols (→Yeast)
- 00:38, 15 August 2011 (hist) (diff) N McClean:Mating Type Testers (New page: ==Overview== How to make mating type tester plates from yMM421 and yMM422 and their drug resistant derivatives. ==Materials== yMM421/422 colonies growing on YPD YPD and/or Drug plates dH<...) (top)
- 00:28, 15 August 2011 (hist) (diff) McClean:Basic Chemostat Guide (top)
- 00:12, 15 August 2011 (hist) (diff) McClean:Basic Chemostat Guide
- 23:05, 14 August 2011 (hist) (diff) McClean:Chemostat Metabolic Cycle (top)
- 12:42, 14 August 2011 (hist) (diff) McClean:Protocols (→Yeast)
- 12:41, 14 August 2011 (hist) (diff) McClean:Chemostat Metabolic Cycle
- 12:30, 14 August 2011 (hist) (diff) N McClean:Chemostat Metabolic Cycle (New page: ==Overview== This is a guide on how to induce the Metabolic Cccle in FY diploid Yeast Strains (First used on 8/5/11-8/14/11) and subsequently sample the culture for various things. To see...)
- 12:27, 14 August 2011 (hist) (diff) McClean:Basic Chemostat Guide
- 12:27, 14 August 2011 (hist) (diff) McClean:Protocols (→Yeast)
- 12:25, 14 August 2011 (hist) (diff) McClean:Basic Chemostat Guide
- 12:18, 14 August 2011 (hist) (diff) McClean:Protocols
- 12:17, 14 August 2011 (hist) (diff) N 96-well plate.pdf (96-well plate.pdf moved to McClean:96-well plate: Not put in right index) (top)
- 12:17, 14 August 2011 (hist) (diff) m McClean:96-well plate (96-well plate.pdf moved to McClean:96-well plate: Not put in right index) (top)
- 12:16, 14 August 2011 (hist) (diff) N McClean:96-well plate (New page: Use this sheet in Lab Notebooks to annotate reaction conditions, strains, or glycerol stock identities, etc. Contains 2 8 (A-H) x 12 (1-12) grids that can be printed out and taped in. [[...)
- 12:10, 14 August 2011 (hist) (diff) N Image:96-well plate.pdf (A PDF that has a grid of a 96 well plate to print and use in lab notebooks.) (top)
- 19:23, 13 August 2011 (hist) (diff) McClean:FISH (→Protocol) (top)
- 12:43, 10 August 2011 (hist) (diff) McClean:Basic Chemostat Guide (→Vessels)
- 12:36, 10 August 2011 (hist) (diff) McClean:Basic Chemostat Guide
- 12:23, 10 August 2011 (hist) (diff) N McClean:Basic Chemostat Guide (New page: ==Overview== This is a simple introduction into how to set up a chemostat experiment using the Botstein Lab Chemostat Room. Adapted from the see [http://www.princeton.edu/genomics/botstei...)
- 19:40, 8 August 2011 (hist) (diff) McClean
- 19:37, 8 August 2011 (hist) (diff) McClean:Protocols (→PCR)
- 19:36, 8 August 2011 (hist) (diff) N McClean:dNTPs (New page: We use Roche Diagnostics dNTP set. Each individual dNTP comes as 10 μmol in 100 mM solution for a total of 4 tubes. Each lids labeled with the nucleic acid it contains. The kits are ...) (top)
- 13:51, 27 July 2011 (hist) (diff) McClean:Protocols
- 13:50, 27 July 2011 (hist) (diff) McClean:LFA Membranes
- 13:50, 27 July 2011 (hist) (diff) McClean:LFA Membranes
- 13:49, 27 July 2011 (hist) (diff) McClean:LFA Membranes (→Final Composition of Media (100 mL total))
- 13:42, 27 July 2011 (hist) (diff) N McClean:LFA Membranes (New page: ==Overview== Use this protocol to make small Low Fluorescence Agar Membranes. Adapted from Wong et al (2010) for use with the FCS2. We adapted the original protocol for bacterial membran...)
- 13:34, 27 July 2011 (hist) (diff) McClean:Protocols
- 11:55, 26 July 2011 (hist) (diff) N McClean:Scope Settings (New page: ==Filter Settings== There are now and additional 2 sets of filters that have been installed for FRET. To use FRET imaging there are YFP and CFP Excitation and EMission Filters that must be...) (top)
- 13:10, 24 July 2011 (hist) (diff) N McClean: Dilution of Oligos (New page: Dilution of Oligos *We order most oligos from IDT[http://www.idtdna.com/Home/Home.aspx|IDT]. *When you receive the oligos, the IDT Spec Sheet will tell you the amount of oligo in nMoles *L...)
- 13:06, 24 July 2011 (hist) (diff) McClean:Protocols
- 13:05, 24 July 2011 (hist) (diff) McClean:Protocols
- 13:04, 24 July 2011 (hist) (diff) N McClean:Touchdown PCR (New page: ==Background== Use this PCR to avoid primers amplifying nonspecific sequences. The procedure is based off of 2 principles. First, the specificity of the products is based off the annealing...) (top)
- 12:27, 8 July 2011 (hist) (diff) McClean:Protocols
- 12:27, 8 July 2011 (hist) (diff) N McClean:Random Spore Prep (New page: Category:Template <!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! --> ==Overview== A way to enrich for haploid cells from a mix of haploids and diploids. This is an alte...)
- 12:03, 8 July 2011 (hist) (diff) McClean:Research (top)
- 12:02, 8 July 2011 (hist) (diff) N McClean:Research (New page: Projects ==Signal Processing by the Yeast Hyperosmotic Response Pathway== How quickly and accurately information propagates through a signaling pathway determines how it functions within ...)
- 13:25, 30 June 2011 (hist) (diff) McClean:Protocols
- 13:22, 30 June 2011 (hist) (diff) McClean:Protocols
- 13:21, 30 June 2011 (hist) (diff) N McClean:Protocols (New page: Back to McClean Lab)
- 13:20, 30 June 2011 (hist) (diff) McClean
- 13:20, 30 June 2011 (hist) (diff) McClean
- 16:43, 28 June 2011 (hist) (diff) McClean:LFM Recipe
- 16:07, 28 June 2011 (hist) (diff) N McClean:LFM Recipe (New page: Standard media except with low-fluorescence yeast nitrogen base. (Yeast nitrogen base without riboflavin and folic acid.) Final Desired Composition of Yeast Nitrogen Base (Note: Ca¬2Cl...)
- 14:55, 28 June 2011 (hist) (diff) User:Anjali Bisaria/Notebook/YMC (→Project Description/Abstract) (top)
- 14:54, 28 June 2011 (hist) (diff) User:Anjali Bisaria/Notebook/YMC/2011/04/19 (→Entry title) (top)
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