User contributions for David Roche
From OpenWetWare
Jump to navigationJump to search
4 September 2009
- 07:4007:40, 4 September 2009 diff hist 0 File:II09 FullSystem.png uploaded a new version of "Image:II09 FullSystem.png" current
- 07:3807:38, 4 September 2009 diff hist 0 File:II09 FullSystem.png uploaded a new version of "Image:II09 FullSystem.png"
- 07:3407:34, 4 September 2009 diff hist 0 N File:II09 FullSystem.png No edit summary
- 07:3407:34, 4 September 2009 diff hist +50 N IGEM:IMPERIAL/2009/Full System New page: === Full System === Image:II09_FullSystem.png current
- 07:3307:33, 4 September 2009 diff hist +2 IGEM:IMPERIAL/2009/4Sep →Agenda
- 07:3307:33, 4 September 2009 diff hist +35 IGEM:IMPERIAL/2009/4Sep →Agenda
- 06:1706:17, 4 September 2009 diff hist +7 IGEM:IMPERIAL/2009/4Sep →Agenda
- 06:1706:17, 4 September 2009 diff hist +51 IGEM:IMPERIAL/2009/4Sep →Agenda
- 05:3605:36, 4 September 2009 diff hist 0 N File:II09 M1a C3.jpg No edit summary current
- 05:3505:35, 4 September 2009 diff hist +27 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated →Module 1 current
- 05:3505:35, 4 September 2009 diff hist +4 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated →Module 0
- 05:3405:34, 4 September 2009 diff hist −1 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated →Module 0
- 05:3405:34, 4 September 2009 diff hist 0 N File:II09 M0 C2.jpg No edit summary current
- 05:3305:33, 4 September 2009 diff hist +37 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated →Module 0
- 05:3105:31, 4 September 2009 diff hist +27 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated No edit summary
- 05:2905:29, 4 September 2009 diff hist +24 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated No edit summary
- 05:2805:28, 4 September 2009 diff hist 0 N File:II09 C1.jpg No edit summary current
- 05:2805:28, 4 September 2009 diff hist −1 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated No edit summary
- 05:2405:24, 4 September 2009 diff hist 0 N File:II09 M0a.png No edit summary current
- 05:2405:24, 4 September 2009 diff hist +50 N IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated New page: Testing Constructs Updated Image:II09_M0a.png
3 September 2009
- 08:4408:44, 3 September 2009 diff hist 0 N File:II09 logoidea1.png No edit summary current
- 08:4408:44, 3 September 2009 diff hist +28 N IGEM:IMPERIAL/2009/Wiki Logo/i New page: Image:II09_logoidea1.png
- 03:3103:31, 3 September 2009 diff hist 0 N File:II09 Temp2.png No edit summary current
- 03:3103:31, 3 September 2009 diff hist +24 N IGEM:IMPERIAL/2009/Wiki Templates/ii New page: Image:II09_Temp2.png current
- 03:3003:30, 3 September 2009 diff hist 0 N File:II09 Temp1.png No edit summary current
- 03:3003:30, 3 September 2009 diff hist +4 IGEM:IMPERIAL/2009/Wiki Templates/i No edit summary current
- 03:2903:29, 3 September 2009 diff hist +20 N IGEM:IMPERIAL/2009/Wiki Templates/i New page: Image:II09_Temp1
2 September 2009
- 02:5502:55, 2 September 2009 diff hist +203 N Calendar:ImperialWetLabNotebook2009iGEM/2009-9-2 New page: ~~~~ <br> AM : Ran a gel with the WaaL pcr products that were digested overnight. 20ul against the ladder <br> Updated wiki and BioBrick registry
1 September 2009
- 05:4005:40, 1 September 2009 diff hist +41 IGEM:IMPERIAL/2009/M2 →Cloning strategy (assembly) current
24 August 2009
- 07:5307:53, 24 August 2009 diff hist +19 IGEM:IMPERIAL/2009/Encapsulation/Modelling/Timer2 →Diagram current
- 07:5207:52, 24 August 2009 diff hist +125 IGEM:IMPERIAL/2009/Encapsulation/Modelling/Timer2 →Diagram
- 07:4907:49, 24 August 2009 diff hist +211 IGEM:IMPERIAL/2009/Encapsulation/Modelling/Timer2 →Model
- 07:4107:41, 24 August 2009 diff hist +240 IGEM:IMPERIAL/2009/Encapsulation/Modelling/Timer2 →Diagram
- 07:2107:21, 24 August 2009 diff hist +8 m IGEM:IMPERIAL/2009/Encapsulation/Modelling/Timer1 →Operations current
- 07:2107:21, 24 August 2009 diff hist +184 IGEM:IMPERIAL/2009/Encapsulation/Modelling/Timer1 →Operations
- 06:2806:28, 24 August 2009 diff hist −4 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-24 →WaaL ligase PCR current
- 06:2806:28, 24 August 2009 diff hist +454 N Calendar:ImperialWetLabNotebook2009iGEM/2009-8-24 New page: ~~~~<br> ===WaaL ligase PCR === <br> <br> * Used Barnes buffer instead of Pfu Ultra Buffer * PCR taken at 48'C, 50'C and 52'C with the negative at 48'C * Successful PCR (at last) at all 3...
21 August 2009
- 07:3707:37, 21 August 2009 diff hist +1 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-21 →Gel Extraction of OtsA, RcsB, Dam current
- 07:3607:36, 21 August 2009 diff hist −49 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-21 →Gel Extraction of OtsA, RcsB, Dam
- 07:3507:35, 21 August 2009 diff hist +389 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-21 →Gel Extraction of OtsA, RcsB, Dam
- 07:3107:31, 21 August 2009 diff hist +66 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-21 No edit summary
- 07:3007:30, 21 August 2009 diff hist +828 N Calendar:ImperialWetLabNotebook2009iGEM/2009-8-21 New page: === WaaL Ligase PCR === <br> <i> 2nd attempt, as it did not work yesterday </i> <br> PCR was using:<br> * TOP10 e-coli genome. * Pfu II Ultra Enzyme * Pfu II Ultra Buffer * dNTPs, fwd and ...
19 August 2009
- 09:2709:27, 19 August 2009 diff hist +1 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 →PAH Site-Directed Mutagenesis
- 09:2609:26, 19 August 2009 diff hist +107 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 →PAH Site-Directed Mutagenesis
- 09:1709:17, 19 August 2009 diff hist +66 IGEM:IMPERIAL/2009/M2/Assays →Assay 3.2: Trehalose Functionality current
- 09:0809:08, 19 August 2009 diff hist +437 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 →Results of Gel
- 09:0509:05, 19 August 2009 diff hist +823 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 →PAH Site-Directed Mutagenesis
- 08:5608:56, 19 August 2009 diff hist +46 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 →PAH Site-Directed Mutagenesis
- 08:5508:55, 19 August 2009 diff hist +281 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 →PAH Site-Directed Mutagenesis
- 08:5308:53, 19 August 2009 diff hist +411 N Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 New page: ~~~~ === PAH Site-Directed Mutagenesis === <i> There are 2 sites in PAH that require site directed mutagenesis (one XbaI site and one PstI site). We chose to start with the XbaI site, ...
