User contributions for Skim
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22 August 2007
9 August 2007
- 14:3814:38, 9 August 2007 diff hist −1 IGEM:MIT/2007/Notebook/2007-8-9 →Accomplished Today current
- 14:3714:37, 9 August 2007 diff hist +7 IGEM:MIT/2007/Notebook/2007-8-9 →Miniprepped Standard Assembly LCs
- 14:3714:37, 9 August 2007 diff hist +4 IGEM:MIT/2007/Notebook/2007-8-9 →Miniprepped Standard Assembly LCs
- 14:3614:36, 9 August 2007 diff hist +908 N IGEM:MIT/2007/Notebook/2007-8-9 New page: ===Accomplished Today=== ==Miniprepped Standard Assembly LCs== *12 tubes, 4 each of CPX/B0014, I14032/B0034 and R0051/B0034, all in DH5alpha *NOTE: Elution with Tris EDTA, not water *Nanod...
7 August 2007
- 13:0913:09, 7 August 2007 diff hist +175 IGEM:MIT/2007/Notebook/2007-8-7 No edit summary current
30 July 2007
- 12:5812:58, 30 July 2007 diff hist +8 IGEM:MIT/2007/Notebook/2007-7-30 →Live Cell Immunoassay (Eric)
- 11:3111:31, 30 July 2007 diff hist +31 IGEM:MIT/2007/Notebook/2007-7-30 →Live Cell Immunoassay (Eric)
- 11:3011:30, 30 July 2007 diff hist +665 IGEM:MIT/2007/Notebook/2007-7-30 No edit summary
24 July 2007
- 13:4413:44, 24 July 2007 diff hist +537 IGEM:MIT/2007/Notebook/2007-7-24 →AGENDA
- 13:3713:37, 24 July 2007 diff hist +685 IGEM:MIT/2007/Notebook/2007-7-24 →AGENDA
- 06:3906:39, 24 July 2007 diff hist +548 N IGEM:MIT/2007/Notebook/2007-7-24 New page: ==AGENDA== *Plate the Mer/I13500 3A transformation after overnight ligation (and grow) *Check if sequence results are available from Monday (length was correct) *Second polystyrene assay (...
23 July 2007
- 12:3512:35, 23 July 2007 diff hist +19 IGEM:MIT/2007/Notebook/2007-7-23 →3A Ligation of Mer promoter+Mer regulatory protein (pTO40) and RBS+GFP (I13500) into pSB1AT3 (SK, JH, BH)
- 12:3412:34, 23 July 2007 diff hist 0 IGEM:MIT/2007/Notebook/2007-7-23 →Agenda
- 12:3312:33, 23 July 2007 diff hist −1 IGEM:MIT/2007/Notebook/2007-7-23 →Agenda
- 12:3212:32, 23 July 2007 diff hist +37 IGEM:MIT/2007/Notebook/2007-7-23 →3A Ligation of Mer promoter+Mer regulatory protein (pTO40) and RBS+GFP (I13500) into pSB1AT3
- 12:3012:30, 23 July 2007 diff hist +319 IGEM:MIT/2007/Notebook/2007-7-23 →3A Ligation of Mer promoter+Mer regulatory protein (pTO40) and RBS+GFP (I13500) into pSB1AT3
- 12:2612:26, 23 July 2007 diff hist +800 IGEM:MIT/2007/Notebook/2007-7-23 →Digestion of I13500
20 July 2007
- 12:2912:29, 20 July 2007 diff hist +45 IGEM:MIT/2007/Notebook/2007-7-20 →Agenda current
- 12:2812:28, 20 July 2007 diff hist +13 IGEM:MIT/2007/Notebook/2007-7-20 →Agenda
- 12:2812:28, 20 July 2007 diff hist +227 IGEM:MIT/2007/Notebook/2007-7-20 →Agenda
19 July 2007
- 08:4508:45, 19 July 2007 diff hist +398 IGEM:MIT/2007/Notebook/2007-7-19 →Agenda for Thursday, 7/19
18 July 2007
- 12:3212:32, 18 July 2007 diff hist +43 IGEM:MIT/2007/Notebook/2007-7-19 →Agenda for Thursday, 7/19
- 12:3012:30, 18 July 2007 diff hist +6 IGEM:MIT/2007/Notebook/2007-7-19 →Agenda for Thursday, 7/19
- 12:3012:30, 18 July 2007 diff hist +61 N IGEM:MIT/2007/Notebook/2007-7-19 New page: ===Agenda for Thursday, 7/19=== *Plating (K+/A+)? *AK/AL list
- 09:4809:48, 18 July 2007 diff hist 0 IGEM:MIT/2007/Notebook/2007-7-18 →Agenda
- 09:4709:47, 18 July 2007 diff hist +385 IGEM:MIT/2007/Notebook/2007-7-18 →Agenda
17 July 2007
- 09:0809:08, 17 July 2007 diff hist +419 IGEM:MIT/2007/Notebook/2007-7-17 →Digestion of (F2620 and B0034) for 3A
14 July 2007
- 23:4423:44, 14 July 2007 diff hist +1 IGEM:MIT/2007/Notebook/2007-7-14 →Weekend Update
- 23:4323:43, 14 July 2007 diff hist +28 IGEM:MIT/2007/Notebook/2007-7-14 →Late Friday (7/13) Afternoon Update
- 23:4323:43, 14 July 2007 diff hist +1,908 N IGEM:MIT/2007/Notebook/2007-7-14 New page: ===Late Friday (7/13) Afternoon Update=== *BH/SK put dry pT040 (?) plates in warm room (for AK and AL) *Tom stopped by -- we have a BUNCH of chemically competent cells in the -80 degree fr...
11 July 2007
- 11:0411:04, 11 July 2007 diff hist +443 IGEM:MIT/2007/Room Reservations No edit summary
9 July 2007
- 10:4810:48, 9 July 2007 diff hist +1 IGEM:MIT/2007/Notebook/2007-7-3 →Incubated plates of DH5a current
- 08:0408:04, 9 July 2007 diff hist +4 IGEM:MIT/2007/Notebook/2007-7-3 →Make LB+KAN agar plates
7 July 2007
- 14:4314:43, 7 July 2007 diff hist +458 IGEM:MIT/2007/Notebook/2007-7-7 →Checked Plates 5 PM current
2 July 2007
- 14:3514:35, 2 July 2007 diff hist +37 IGEM:MIT/2007/Notebook/2007-7-3 →Agenda for July 3
- 14:3514:35, 2 July 2007 diff hist +41 IGEM:MIT/2007/Notebook/2007-7-3 →Agenda for July 3
- 14:3414:34, 2 July 2007 diff hist +140 N IGEM:MIT/2007/Notebook/2007-7-3 New page: ===Agenda for July 3=== *Call Geneart *Results of Alex/Diti PCR? *For metals: RBS and promoter? (clarify!) *Barry's promoter -- obtainment?
1 July 2007
29 June 2007
- 13:2513:25, 29 June 2007 diff hist +39 N IGEM:MIT/2007/Notebook/2007-7-2 New page: ===Agenda: Monday, 7/2=== *Order stuff!
28 June 2007
- 22:3022:30, 28 June 2007 diff hist +507 N IGEM:MIT/2007/Notebook/2007-6-29 New page: ===Agenda for Friday, June 29=== *Morning **pBAD pickup **Is OmpA crucial to bacterial cell survival? **Finalize oligos (restriction sites) **Research "red team" stuff -- ion exchange **Br...
25 June 2007
- 12:1712:17, 25 June 2007 diff hist +412 N Talk:IGEM:MIT/2007/Notebook/2007-6-25 New page: ===CHECKLIST for Monday, 6/25=== *Reorganize wiki *CPX/OmpC inserts **Polystyrene 17 **Polystyrene 18 (Belcher?) **Polypyrrole *Check sequences for CPX *Call Brian/grads/Eric check inserts... current
- 12:0512:05, 25 June 2007 diff hist +686 N IGEM:MIT/2007/Notebook/2007-6-25 New page: ===USING SEQUENCHER (Demo)=== *Copy desired gene sequence (to check) *Sequence tab **Create New Sequence **Give name **Paste sequence *View tab **Translation – different reading frame...
20 June 2007
- 07:5007:50, 20 June 2007 diff hist +742 IGEM:MIT/2007/Ideas3 →Mussel Protein
17 June 2007
- 21:4721:47, 17 June 2007 diff hist +1 IGEM:MIT/2007/Notebook/2007-6-17 →Notes from Meeting at 8 pm current
- 21:4621:46, 17 June 2007 diff hist +2,971 IGEM:MIT/2007/Notebook/2007-6-17 No edit summary
11 June 2007
- 20:1120:11, 11 June 2007 diff hist 0 IGEM:MIT/2007/Notebook/2007-6-11 →Making Plates
- 20:1120:11, 11 June 2007 diff hist −2 IGEM:MIT/2007/Notebook/2007-6-11 →Transforming DNA plasmids into bacterial colonies on plates
- 20:0920:09, 11 June 2007 diff hist −2 IGEM:MIT/2007/Notebook/2007-6-11 →General Lab Do s and Don’t s
- 20:0820:08, 11 June 2007 diff hist −10 IGEM:MIT/2007/Notebook/2007-6-11 →General Lab Do s and Don’t s