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- 18:42, 16 April 2024 Nanoimprint Lithography (NIL) - Carter Paul (hist | edit) [7,205 bytes] CarterPaul (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} =Motivation= =Introduction to NIL= =Thermal NIL Process=")
- 18:40, 16 April 2024 3D Cell Culture - McLean Taggart, Emma Villares, Maximillian Marek, Scott LeBlanc, Adam Lyons and Jacob Belden (hist | edit) [24,057 bytes] CarterPaul (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} ==Introduction== While most microfluidic devices incorporate a 2D cell culture design, in which a single layer of cells is grown on the bottom of a device, these systems suffer from poor <i>in vivo</i> mimicry, as, in the human body, most cells grow in all directions.<sup>https://doi.org/10.5114/aoms.2016.63743 1</sup> To address this limitation, 3D cell culture devices have been developed - in w...")
- 17:03, 15 April 2024 Multilayer Paper Microfluidics - Madyson Redder (hist | edit) [6,228 bytes] Mredder (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} Overview 3D polymeric or glass microfluidic devices were created to run tests on small amounts of liquid and receive results in a timely manner. However, these devices are costly and time consuming to produce. A solution to this problem was single-layer paper microfluidic devices. The most common known examples of single-layer paper microfluidic devices are pregnancy tests, COVID-19 antigen tests, and glucose test strips. While these devices a...")
- 10:33, 14 April 2024 The paper that launched microfluidics - Xi Ning (hist | edit) [11,843 bytes] Xning098 (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} Microfluidic devices are labs on the chip or microchemical systems or with various applications such as models, reactors, and detectors. They have many benefits that larger scale systems can not achieve. The paper by Harrison et al. is widely recognized as one to establish and popularize microfluidics as a research field, pioneering the phrase lab on a chip and showing its feasibility. <sup>https://doi.org/10.1039/d3lc90076b 2</sup> During...")
- 12:10, 13 April 2024 Cells and Nanoparticles in Flow - Namish Kokkula (hist | edit) [6,194 bytes] Nkokkula (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} == Introduction == == References ==")
- 17:42, 12 April 2024 Free-Boundary Microfluidics - Robert Keane (hist | edit) [22,343 bytes] Rkeane (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} == Introduction: Types of Free-Boundary Microfluidics == == Hydrodynamic == Flow Focusing, Co-flow, Cross flow == Elastohydrodynamics == Electrospray, EHD, Electrospinning == Interfacial Tension == Multi-axial, Oblique, Spinning == Acoustics == Ejection, Acoustophoretic, Vibrating == References == 1.")
- 12:01, 10 April 2024 Microfluidic Vasculature for Cell Culture - Evelyn Moore (hist | edit) [8,282 bytes] Ejmoore (talk | contribs) (Created blank page)
- 07:10, 10 April 2024 Combining Body on a Chip - Recreating the Tumor Microenvironment - Organ on a Chip - Michele Caggioni (hist | edit) [57,785 bytes] Michele Caggioni (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}} ==Introduction== Body-on-a-chip, [https://openwetware.org/wiki/Organ-on-a-chip_-_Dan_Nguyen Organ-on-a-chip], and Tumor-on-a-chip all represent a consolidation of microfluidic technologies and biological practices in efforts to model body processes on a higher level of detail and complexity. Through the development of Lab-on-a-chip devices, these microfluidic devices have opened a door that could allow for more human-specific research to occur....")
- 11:22, 9 April 2024 Microfluidics for DNA Sequencing - Khiem Le (hist | edit) [13,151 bytes] Khiemle (talk | contribs) (Created page with "{{Template:CHEM-ENG590E}}")
- 11:20, 2 April 2024 DropBase:Picoinjection chip (hist | edit) [411 bytes] Florian Hollfelder (talk | contribs) (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= '''Description''' Sorter and FF devives. <br> '''Reference''' Selection of a Promiscuous Minimalist cAMP Phosphodiesterase from a Library of De Novo Designed Proteins D.Schnettler et al, bioRxiv, 2023, DOI: 10.1101/2023.02.13.528392 <br> <br> =Downloads= <ol style="list-style-type: lower-roman;"> <li> Chips; dwg <br></li></ol>")
- 11:14, 2 April 2024 DropBase:Sorter and FF devices (hist | edit) [459 bytes] Florian Hollfelder (talk | contribs) (Created page with "=Overview= '''Description''' Sorter and Flow Focussing devices. <br> '''Reference''' Nikolic, N., Anagnostidis, V., Tiwari, A., Chait, R. & Gielen, F. Investigating bacteria-phage interaction dynamics using droplet-based technology. BioRxiv, doi:10.1101/2023.07.14.549014 (2023) <br> <br>")
- 07:39, 2 April 2024 UA Biophysics:Protocols:Elution Buffer ESP (hist | edit) [940 bytes] Elizabeth Suesca (talk | contribs) (Created page with " ==Procedimiento== # Colocar 1.6 litros de agua deionizada en la botella con un agitador. # Diluir el HEPES (concentracion final de 20 mM) y el NaCl (concnetracion final de 170 mM). # Ajustar pH a 7.4 usando NaOH y/o HCl. # Determinar la concentración de HCl y NaOH. Por ejemplo, si entre los dos se agregaron 16 ml a 1M, entonces para un buffer de 2 L la concentración es de 8 mM. # Calcular la osmolaridad y completar los 400 mOsm con NaCl. # Sacar el agitador y co...")
- 00:31, 26 March 2024 Orgenizing the data from the LCMS mechine (hist | edit) [410 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "* In order to organize the data for further analysis , we use script in R * Please go over the presentations File:presentation1.ppt, File:presentation2.ppt, File:presentation2.ppt")
- 11:02, 25 March 2024 Designing and testing the qPCR primers (hist | edit) [4,641 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "# For additional information [https://www.dropbox.com/scl/fi/070fhrrw6heimv1xxuxzq/real-time-pcr-handbook-life-technologies-update-flr.pdf?rlkey=eddim7471x9l8uqww1234yg6w&dl=0] ===House keeping genes=== # When checking gene expressed in the BSF it is important to use housekeeping gene as a reference. # Housekeeping genes, also known as reference genes, are a set of genes that are constitutively expressed in cells and are essential for basic cellular functions. # T...")
- 14:34, 14 February 2024 UA Biophysics: Extruder Cleaning Instructons ESP (hist | edit) [601 bytes] Elizabeth Suesca (talk | contribs) (Created page with "Tenga en cuenta que nunca se deben sonicar los émbolos. #Enjuagar con abundante agua los tambores, jeringas y el cilindro metálico #Sonicar con agua desionizada los tambores (10 minutos), si nota que el agua sale muy verde, cambie el agua y sonique nuevamente. #Sonicar con etanol 95% los tambores (10 minutos), #Purgar 10 veces las jeringas conectadas a los tambores con agua desionizada y 10 veces más con alcohol al 95%. #En caso de que no se logre limpiar por comple...")
- 08:54, 12 February 2024 DropBase:sorter and FF devices (hist | edit) [416 bytes] Florian Hollfelder (talk | contribs) (Created page with "=Overview= '''Description''' Flow focussing chip and dielectrophoretic sorter <br> '''Reference''' Nikolic, N., Anagnostidis, V., Tiwari, A., Chait, R. & Gielen, F. Investigating bacteria-phage interaction dynamics using droplet-based technology. BioRxiv, doi:10.1101/2023.07.14.549014 (2023) <br> <br>")
- 12:08, 6 February 2024 Misb23 (hist | edit) [24,700 bytes] Pablo Carbonell (talk | contribs) (Created page with "= Computational Enzyme Design: A Practical Exercise = Pablo Carbonell, [https://www.upv.es/ Universitat Politècnica de València] == Objectives == In this exercise, you will learn about bioinformatics tools and databases that can guide you in order to perform an ''in silico'' protein design. New tools are continuously becoming available. It is advisable, thus, to check always for the latest developments. For instance, take a look [http://www.ncbi.nlm.nih.gov/pubmed?t...")
- 12:48, 5 February 2024 McClean: Tester Protocol (hist | edit) [8,210 bytes] Megan N McClean (talk | contribs) (Created page with "# **EMSA Protocol** **Overview** This is a protocol for the LightShift Chemiluminescent EMSA Kit from Thermo Scientific (cat # 20148). It is highly recommended to read the instructions that come with the kit. They have a lot of good information that will help with decision making. **Materials** - LightShift EMSA Optimization and Control Kit (20148X) - Chemiluminescent Nucleic Acid Detection Module (89880) - Biotin 3' or 5' end-labeled DNA target - Unlabeled DNA targ...")
- 08:50, 28 January 2024 Checking the RNA integrity on gel electrophoresis (hist | edit) [1,705 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "===Preparation of the gel=== * Prepare a 1.5-2% gel electrophoresis. * For example, for a small gel, add 0.53 g agarose and 35 ml TBE/TAE to an Erlenmeyer flask. * Microwave for 30-45 seconds, stop and swirl, and then continue towards a boil until the agarose is completely dissolved. * Let the agarose solution cool down and add 3 µl of 'redsafe' (the amount depends on the 'redsafe' company). * Pour the agarose into a gel tray with the well comb in place. * Use the s...")
- 08:39, 28 January 2024 How to interpret nano-drop results (hist | edit) [786 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "===260/280 Ratio=== * The ratio of absorbance at 260 nm and 280 nm is used to assess the purity of DNA and RNA. * A ratio of ~2.0 is generally accepted as 'pure' for RNA. * If the ratio is appreciably lower in either case, it may indicate the presence of protein, phenol, or other contaminants that absorb strongly at or near 280 nm. ===260/230 Ratio=== *This ratio is used as a secondary measure of nucleic acid purity. * Expected 260/230 values are commonly in the ra...")