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- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/20 (category OWWLabNotebookV1)VF2 (diluted 10 times from the stock tube) : 2,5µL PCR MM 2X : 25 µL water : 20 µL Here is the PCR pgm : 95°C - 15' (to lyse cells too) 95°C - 30" 50°C -...4 KB (0 words) - 22:01, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/02 (category OWWLabNotebookV1)volume (µL) Plates L1 10 20 200µL pur Cm L2 10 20 200µL pur Cm L3 10 20 200µL pur positive transformation control Cm (pSB1C3) 1 20 200µL Cm negative Cm transformation...4 KB (0 words) - 21:56, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/16 (category OWWLabNotebookV1)a transformation of the BB with the backbone pSB1T3. I transformed 2µL in 20µL of cell. I recover from -80°C stock : pSB4K5 for the QCM quick change pSC013/014...1 KB (0 words) - 21:59, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/18 (category OWWLabNotebookV1)that carry an amber suppressor. I transformed 4 times from each sample 5µL in 20µL of cell. The positive control is the plasmid I used before the PCR. So for...2 KB (0 words) - 22:00, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/05 (category OWWLabNotebookV1)amber suppressor in their genome (supE44, or glnV44). I re-transform 2µL in 20µL of competent MG1655 from Jake's stock, and I plate 200µL on LBA+Amp. The...2 KB (0 words) - 21:57, 26 September 2017